The lin-41 RBCC gene acts in the C-elegans heterochronic pathway between the let-7 regulatory RNA and the LIN-29 transcription factor
The lin-41 RBCC gene acts in the C-elegans heterochronic pathway between the let-7 regulatory RNA and the LIN-29 transcription factor
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DOI：
10.1016/s1097-2765(00)80245-2
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发表时间：
2000-04-01
期刊：
影响因子：
16
通讯作者：
Ruvkun, G
中科院分区：
文献类型：
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作者：
Slack, FJ;Basson, M;Ruvkun, G
C.线虫异时基因lin-41在幼虫期引起成虫fate的早熟表达。lin-41活性的增加导致相反的表型，幼虫命运的重复。let-7突变引起类似的重复异时表型，其被lin-41突变抑制，表明lin-41受let-7负调控。lin-41负调节LIN-29成人特化转录因子表达的时间。lin-41编码RBCC蛋白，并且lin-41 3 'UTR中的两个元件与21个核苷酸的let-7调节RNA互补。lin-41：：GFP融合基因在受lin-41影响的组织中下调，同时let-7调节RNA上调。我们认为，晚期幼虫激活let-7 RNA表达下调LIN-41，以减轻抑制lin-29。
Null mutations in the C. elegans heterochronic gene lin-41 cause precocious expression of adult fates at larval stages. Increased lin-41 activity causes the opposite phenotype, reiteration of larval fates. let-7 mutations cause similar reiterated heterochronic phenotypes that are suppressed by lin-41 mutations, showing that lin-41 is negatively regulated by let-7. lin-41 negatively regulates the timing of LIN-29 adult specification transcription factor expression. lin-41 encodes an RBCC protein, and two elements in the lin-41 3'UTR are complementary to the 21 nucleotide let-7 regulatory RNA. A lin-41::GFP fusion gene is downregulated in the tissues affected by lin-41 at the time that the let-7 regulatory RNA is upregulated. We suggest that late larval activation of let-7 RNA expression downregulates LIN-41 to relieve inhibition of lin-29.