Noninvasive monitoring and tracking of muscle stem cell transplants
Noninvasive monitoring and tracking of muscle stem cell transplants
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DOI：
10.1097/01.tp.0000145528.51525.8b
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发表时间：
2004-12-15
期刊：
影响因子：
6.2
通讯作者：
Walter, GA
中科院分区：
文献类型：
--
作者：
Cahill, KS;Gaidosh, G;Walter, GA
背景。有效的无创监测干细胞移植的技术将加速干细胞治疗的发展。标记干细胞的磁共振成像是一种无创的方法，可以提供高空间分辨率的图像。在本报告中，我们评估了一种市售的、fda批准的造影剂用于监测治疗性干细胞移植小鼠肌营养不良的能力。用阿魏氧化铁：多阳离子复合物孵育，标记多能性肌肉源性干细胞。在体外和体内对标记干细胞分化为成熟肌管的能力进行了评估，并通过高分辨率磁共振成像进行了检测。内体超顺磁性氧化铁积聚导致MR对比T-2和T-2*的变化，允许对标记细胞进行三维，无创检测。对细胞幻影的弛豫测量表明，MR成像可以检测到少于8个标记细胞。此外，将治疗性细胞移植物移植到营养不良的肌肉中可以连续成像，这些非侵入性图像与常规显微镜获得的组织学图像相关。进一步的研究表明，磁共振成像可用于跟踪动脉输送后少量标记细胞的迁移。磁共振监测是一种高灵敏度的技术，适用于肌肉干细胞移植。我们预计它将通过减少侵入性组织采集和活检的需要来加强干细胞研究。
Background. Efficient techniques to noninvasively monitor stem cell transplants will accelerate the development of stem cell therapies. Magnetic resonance (MR) imaging of labeled stem cells is a noninvasive approach that can provide images with high spatial resolution. In this report, we have evaluated the ability of a commercially available, FDA-approved contrast agent to allow for the monitoring of therapeutic stem cell transplants in murine dystrophic muscle.Methods. Multipotent, muscle-derived stem cells were labeled by incubation with ferumoxide:polycation complexes. Labeled stem cells were evaluated for the ability to differentiate into mature myotubes and be detected by high resolution MR images in vitro and in vivo.Results. Endosomal accumulation of superparamagnetic iron-oxide resulted in changes in the MR contrast T-2 and T-2*, allowing for three dimensional, noninvasive detection of labeled cells. Relaxivity measurements on cell phantoms indicate that less than eight labeled cells could be detected by MR imaging. Furthermore, therapeutic cellular grafts transplanted into dystrophic muscle could be imaged sequentially and these noninvasive images correlated with histological images obtained by conventional microscopy. Additional studies revealed that MR imaging is applicable to tracking the migration of a small number of labeled cells following arterial delivery.Conclusions. MR monitoring is a highly sensitive technique that is applicable to muscle stem cell transplantation. We anticipate that it will enhance stem cell investigations by reducing the need for invasive tissue harvests and biopsies.