Heterogeneity of dynamic shear properties of the meniscus: A comparison between tissue core and surface layers.
Heterogeneity of dynamic shear properties of the meniscus: A comparison between tissue core and surface layers.
复制标题
半月板动态剪切特性的异质性：组织核心层和表层之间的比较。
DOI：
10.1002/jor.25495
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Travascio,Francesco
中科院分区：
文献类型：
--
作者：
Schwartz,Gabi;Morejon,Andy;Gracia,Julissa;Best,ThomasM;Jackson,AliciaR;Travascio,Francesco
半月板的损伤与过大的剪切载荷有关。为了阐明半月板的病理生理机制，以前的研究已经对半月板纤维软骨核心的剪切特性进行了研究。然而，半月板在结构上是不均匀的，外面的软骨膜(胫骨和股骨表层)包裹着组织核心。到目前为止，人们对表层的剪切行为知之甚少。这项研究的目的是测量表层的动态剪切特性，并推导出与其组成的经验关系。标本取自猪半月板的胫骨、股骨表面层和骨核(内侧和外侧各10例，n= )。频率扫描测试得到了复数剪切模量(G*)和相移(δ)。区域的力学行为用广义麦克斯韦模型来描述。研究了切变模量值与组织区域糖胺多糖含量的相关性。与核心和胫骨区域相比，股骨表面的剪切模数最低。3松弛时间麦克斯韦模型令人满意地解释了所有组织区域的剪切行为。组织成分也不均匀，与组织核心相比，表层的水分含量更高。水分含量与各地区的剪切特性呈负相关。测量到的股骨层较低的剪切性能可能解释了组织上表面半月板撕裂发生率较高的原因。组织在剪切中的异质性行为提供了对半月板病理的洞察，并对组织工程替代组织的努力具有重要的意义。
Damage to the meniscus has been associated with excessive shear loads. Aimed at elucidating meniscus pathophysiology, previous studies have investigated the shear properties of the meniscus fibrocartilaginous core. However, the meniscus is structurally inhomogeneous, with an external cartilaginous envelope (tibial and femoral surface layers) wrapping the tissue core. To date, little is known about the shear behavior of the surface layers. The objective of this study was to measure the dynamic shear properties of the surface layers and derive empirical relations with their composition. Specimens were harvested from tibial and femoral surface layers and core of porcine menisci (medial and lateral,n= 10 each). Frequency sweep tests yielded complex shear modulus (G*) and phase shifts (δ). Mechanical behavior of regions was described by a generalized Maxwell model. Correlations between shear moduli with water and glycosaminoglycans content of the tissue regions were investigated. The femoral surface had the lowest shear modulus, when compared to core and tibial regions. A 3‐relaxation times Maxwell model satisfactorily interpreted the shear behavior of all tissue regions. Inhomogeneous tissue composition was also observed, with water content in the surface layers being higher when compared with tissue core. Water content negatively correlated with shear properties in all regions. The lower measured shear properties in the femoral layer may explain the higher prevalence of meniscal tears on the superior surface of the tissue. The heterogenous behavior of the tissue in shear provides insight into meniscus pathology and has important implications for efforts to tissue engineer replacement tissues.