Responses of soil microbial community to continuous experimental nitrogen additions for 13 years in a nitrogen-rich tropical forest
Responses of soil microbial community to continuous experimental nitrogen additions for 13 years in a nitrogen-rich tropical forest
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富氮热带森林土壤微生物群落对连续实验氮添加13年的响应
DOI：
10.1016/j.soilbio.2018.03.009
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发表时间：
2018-06
影响因子：
9.7
通讯作者：
Mo Jiangming
中科院分区：
文献类型：
--
作者：
Wang Cong;Lu Xiankai;Mori Taiki;Mao Qinggong;Zhou Kaijun;Zhou Guoyi;Nie Yanxia;Mo Jiangming
人类活动的加剧将在未来几十年增加氮(N)的沉积速率，特别是在热带地区。迫切需要了解富氮热带森林中的土壤微生物群落对长期氮沉积的反应。在中国南部一片富氮热带森林中，利用正在进行的试验田，研究了长期施氮对土壤微生物量(氯仿熏蒸测定)、微生物群落组成(以磷脂脂肪酸为基础)和微生物酶活性的影响。有4个氮素添加水平：不添加(对照)；50 kg N ha(-1)yr(-1)(低氮)；100 kg N ha(-1)yr(-1)(中N)；150 kg N ha(-1)yr(-1)(高N)。结果表明，长期施氮显著降低了土壤微生物量碳(MBC)和氮(MBN)，但对总PLFAs影响不大。然而，增加N投入显著降低了细菌PLFA的相对丰度，尤其是N处理小区中革兰氏阳性菌/革兰氏阴性菌比率较高的革兰氏阴性菌PLFA。虽然施氮量对菌菌比影响不大，但丛枝菌根真菌所占比例随着施氮量的增加而显著增加。长期施氮显著增加了细菌胁迫指数，提高了与碳、氮、磷矿化相关的特定酶活性(单位微生物生物量的活性)。同时，微生物群落组成和比酶活性的变化与土壤pH和速效N密切相关。这些结果表明，N介导的环境胁迫在微生物群落的形成中起着重要作用，在高N沉积条件下，土壤微生物将投入更多的资源用于富N森林的酶生产。
Intensified anthropogenic activities will increase rates of nitrogen (N) deposition over the next decades, especially in the tropics. There are urgent needs to know how soil microbial community in N-rich tropical forests responds to long-term N deposition. This study examined effects of long-term N additions on soil microbial biomass (determined by chloroform fumigation), microbial community composition (based on phospholipid fatty acids, PLFAs), and microbial enzyme activities, using an ongoing experimental N additions field in an N rich tropical forest of South China. There were four N additions levels: no additions (Control); 50 kg N ha(-1) yr(-1) (Low-N); 100 kg N ha(-1) yr(-1) (Medium-N), and 150 kg N ha(-1) yr(-1) (High-N). Results showed that long-term N additions significantly decreased microbial biomass carbon (MBC) and nitrogen (MBN), but had little effects on total PLFAs. However, elevated N inputs significantly reduced the relative abundance of bacterial PLFAs, especially gram-negative bacterial PLFAs with higher gram-positive bacteria: gram-negative bacteria ratio in N treatment plots. Although N additions did not change fungi: bacteria ratio, the proportion of arbuscular mycorrhizal fungi increased significantly with N additions. Long-term N additions greatly increased bacterial stress indexes and enhanced specific enzyme activity (activity per unit of microbial biomass) involved in carbon, nitrogen and phosphorus mineralization. Meanwhile, shifts in microbial community composition and specific enzyme activity were correlated well with soil pH and available N. These results suggest that N-mediated environmental stresses can play an important role in shaping microbial community, and that soil microbes will invest more resources on enzyme production in N-rich forest under elevated N deposition.
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影响因子：
9.7
作者：
Saiya-Cork, KR;Sinsabaugh, RL;Zak, DR
通讯作者：
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作者：
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作者：
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通讯作者：
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影响因子：
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作者：
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Benjamin L Turner;S. Joseph Wright
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影响因子：
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作者：
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通讯作者：
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