Disruption of the fibroblast growth factor-2 gene results in decreased bone mass and bone formation
Disruption of the fibroblast growth factor-2 gene results in decreased bone mass and bone formation
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DOI：
10.1172/jci8641
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发表时间：
2000-04-01
影响因子：
15.9
通讯作者：
Hurley, MM
中科院分区：
文献类型：
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作者：
Montero, A;Okada, Y;Hurley, MM
碱性成纤维细胞生长因子（FGF-2）是软骨和骨生长和分化的重要调节因子，在成骨细胞中表达和调节。为了研究FGF-2在骨中的作用，我们检查了Fgf 2基因被破坏的小鼠。micro-CT测量Fgf 2（+/+）和Fgf 2（-/-）成年小鼠股骨干骺端的骨小梁结构发现，Fgf 2（-/-）小鼠的板状骨小梁结构明显减少，许多骨小梁连接杆丢失。动态组织形态测定证实骨小梁体积、矿物质沉积和骨形成率显著降低。此外，Fgf 2（-/-）小鼠骨髓基质培养物的矿化程度显著降低。这项研究提供了强有力的证据表明，FGF-2有助于确定骨量以及骨形成。
Basic fibroblast growth factor (FGF-2), an important modulator of cartilage and bone growth and differentiation, is expressed and regulated in osteoblastic cells. To investigate the role of FGF-2 in bone, we examined mice with a disruption of the Fgf2 gene. Measurement of trabecular bone architecture of the femoral metaphysis of Fgf2(+/+) and Fgf2(-/-) adult mice by micro-CT revealed that the platelike trabecular structures were markedly reduced and many of the connecting rods of trabecular bone were lost in the Fgf2(-/-) mice. Dynamic histomorphometry confirmed a significant decrease in trabecular bone volume, mineral apposition, and bone formation rates. Tn addition, there was a profound decreased mineralization of bone marrow stromal cultures from Fgf2(-/-) mice. This study provides strong evidence that FGF-2 helps determine bone mass as well as bone formation.