Small leucine-rich repeat proteoglycans associated with mature insoluble elastin serve as binding sites for galectins
Small leucine-rich repeat proteoglycans associated with mature insoluble elastin serve as binding sites for galectins
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与成熟不溶性弹性蛋白相关的富含亮氨酸的小重复蛋白多糖作为半乳糖凝集素的结合位点
DOI：
10.1080/09168451.2017.1374828
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
N.
中科院分区：
文献类型：
--
作者：
Itoh;A.;Nonaka;Y.;Ogawa;T.;Nakamura;T.;Nishi;N.
我们之前报道了半乳糖凝集素-9 (Gal-9)，一种免疫调节动物凝集素，可以结合不溶性胶原制剂并对免疫细胞产生直接的细胞杀伤作用。在本研究中，我们发现成熟的不溶性弹性蛋白能够结合Gal-9和人类凝集素家族的其他成员。对一系列市售水溶性弹性蛋白制剂PES-(a) ~ PES-(E)的凝集素印迹分析显示，只有PES-(E)含有Gal-9识别的物质。PES-(E)中gal -9相互作用物质经亲和纯化，胰酶消化，反相高效液相色谱分析。通过n端氨基酸序列分析，鉴定了5个富含亮氨酸的小重复蛋白多糖家族成员（versican、lumican、osteoglycin/mimecan、prolargin和fibromodulin）的肽段。结果表明，Gal-9和可能的其他半乳糖凝集素识别与不溶性弹性蛋白相关的富含亮氨酸的小重复蛋白聚糖上的聚糖，也表明成熟的不溶性弹性蛋白可能作为半乳糖凝集素的细胞外储存库。
We previously reported that galectin-9 (Gal-9), an immunomodulatory animal lectin, could bind to insoluble collagen preparations and exerted direct cytocidal effects on immune cells. In the present study, we found that mature insoluble elastin is capable of binding Gal-9 and other members of the human galectin family. Lectin blot analysis of a series of commercial water-soluble elastin preparations, PES-(A) ~ PES-(E), revealed that only PES-(E) contained substances recognized by Gal-9. Gal-9-interacting substances in PES-(E) were affinity-purified, digested with trypsin and then analyzed by reversed-phase HPLC. Peptide fragments derived from five members of the small leucine-rich repeat proteoglycan family, versican, lumican, osteoglycin/mimecan, prolargin, and fibromodulin, were identified by N-terminal amino acid sequence analysis. The results indicate that Gal-9 and possibly other galectins recognize glycans attached to small leucine-rich repeat proteoglycans associated with insoluble elastin and also indicate the possibility that mature insoluble elastin serves as an extracellular reservoir for galectins.