Influences of centrifugation on cells and tissues in liposuction aspirates: Optimized centrifugation for lipotransfer and cell isolation
Influences of centrifugation on cells and tissues in liposuction aspirates: Optimized centrifugation for lipotransfer and cell isolation
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DOI：
10.1097/01.prs.0000299384.53131.87
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发表时间：
2008-03-01
影响因子：
3.6
通讯作者：
Yoshimura, Kotaro
中科院分区：
文献类型：
--
作者：
Kurita, Masakazu;Matsumoto, Daisuke;Yoshimura, Kotaro
背景资料：虽然注射性自体脂肪移植是软组织增强最有吸引力的选择之一，但脂肪坏死导致的不可预测性和纤维化等问题限制了其普遍接受。反冲洗是克服这些困难的最常用方法之一。本研究进行定量研究离心对吸脂吸出物的影响，以优化脂肪移植和分离脂肪干细胞的离心条件。方法：吸脂吸出物，从8名健康女性供体，要么不离心或离心在400，700，1200，3000，或4200克3分钟。检查油、脂肪和液体部分的体积以及每个部分中的血细胞和脂肪来源的细胞的数量。将经处理的脂肪组织（1ml）注射到无胸腺小鼠中，并在4周时收获移植物并称重。形态学改变进行了观察，使用光镜和扫描电镜。结果：冷冻浓缩脂肪组织和脂肪来源的干细胞在脂肪部分和部分去除红细胞从脂肪部分。超过3000 g的冷冻显著损害脂肪来源的干细胞。冷冻增强每1毫升离心脂肪移植，但减少计算每1毫升脂肪前centrifugation.Conclusions：过度离心可以破坏脂肪细胞和脂肪来源的干细胞，但适当的离心浓缩，导致增强移植。作者初步推荐1200 g作为脂肪移植中获得良好短期和长期结果的最佳离心力。
Background: Although injective autologous fat transplantation is one of the most attractive options for soft-tissue augmentation, problems such as unpredictability and fibrosis resulting from fat necrosis limit its universal acceptance. Centrifugation is one of most common methods for overcoming these difficulties. This study was performed to investigate quantitatively the effects of centrifugation on liposuction aspirates to optimize centrifugal conditions for fat transplantation and isolation of adipose-derived stem cells.Methods: Liposuction aspirates, obtained from eight healthy female donors, were either not centrifuged or centrifuged at 400, 700, 1200, 3000, or 4200 g for 3 minutes. The volumes of the oil, adipose, and fluid portions and numbers of blood cells and adipose-derived cells in each portion were examined. The processed adipose tissues (1 ml) were injected into athymic mice, and grafts were harvested and weighed at 4 weeks. Morphologic alterations were observed using light and scanning electron microscopy.Results: Centrifugation concentrated adipose tissues and adipose-derived stem cells in the adipose portion and partly removed red blood cells from the adipose portion. Centrifugation at more than 3000 g significantly damaged adipose-derived stem cells. Centrifugation enhanced graft take per 1 ml centrifuged adipose but reduced calculated graft take per 1 ml adipose before centrifugation.Conclusions: Excessive centrifugation can destroy adipocytes and adipose-derived stem cells, but appropriate centrifugation concentrates them, resulting in enhanced graft take. The authors tentatively recommend 1200 g as an optimized centrifugal force for obtaining good short- and long-term results in adipose transplantation.