Extremely sensitive dual imaging system in solid phantoms.
Extremely sensitive dual imaging system in solid phantoms.
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实体模型中极其灵敏的双成像系统。
DOI：
10.1117/12.2207908
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Ray,Krishanu
中科院分区：
文献类型：
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作者：
Barnoy,EranA;Fixler,Dror;Popovtzer,Rachela;Nayhoz,Tsviya;Ray,Krishanu
在这里，我们描述了荧光寿命成像显微镜(FLIM)和扩散反射(DR)医学成像技术相结合的有希望的结果。制备了三种不同几何形状的金纳米颗粒(GNPs)：直径为20 nm的球体、长径比(AR)为2.5的棒状颗粒和AR为3.3的棒状颗粒。然后使用估计长度为10 nm的聚乙二醇偶联物将每个GNP几何图形连接到3种不同的荧光染料：荧光素、罗丹明B和磺胺红B。DR在固体组织模拟模体内提供深体积测量(高达1 cm)，指示GNR的存在，对应于记录从GNP散射的光所使用的光，随着到光电探测器的距离增加。薄膜成像溶液以及体模表面，记录荧光寿命以及荧光强度。荧光猝灭观察到荧光素，而金属增强荧光(MEF)在罗丹明B和磺胺基若丹明B中观察到，这两种染料的吸收峰波长略长于GNP等离子体共振峰。由于MEF提供的强度增加，我们的系统具有很高的灵敏度，而且由于FLIM和DR的固有敏感性，这两种模式和MEF可以为生物样本的分子和功能成像提供大量有意义的信息。
Herein we describe promising results from the combination of fluorescent lifetime imaging microscopy (FLIM) and diffusion reflection (DR) medical imaging techniques. Three different geometries of gold nanoparticles (GNPs) were prepared: spheres of 20nm diameter, rods (GNRs) of aspect ratio (AR) 2.5, and GNRs of AR 3.3. Each GNP geometry was then conjugated using PEG linkers estimated to be 10nm in length to each of 3 different fluorescent dyes: Fluorescein, Rhodamine B, and Sulforhodamine B. DR provided deep-volume measurements (up to 1cm) from within solid, tissue-imitating phantoms, indicating GNR presence corresponding to the light used by recording light scattered from the GNPs with increasing distance to a photodetector. FLIM imaged solutions as well as phantom surfaces, recording both the fluorescence lifetimes as well as the fluorescence intensities. Fluorescence quenching was observed for Fluorescein, while metal-enhanced fluorescence (MEF) was observed in Rhodamine B and Sulforhodamine B – the dyes with an absorption peak at a slightly longer wavelength than the GNP plasmon resonance peak. Our system is highly sensitive due to the increased intensity provided by MEF, and also because of the inherent sensitivity of both FLIM and DR. Together, these two modalities and MEF can provide a lot of meaningful information for molecular and functional imaging of biological samples.