Coinfection with multiple strains of the Epstein-Barr virus in human immunodeficiency virus-associated hairy leukoplakia.
Coinfection with multiple strains of the Epstein-Barr virus in human immunodeficiency virus-associated hairy leukoplakia.
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DOI：
10.1073/pnas.89.14.6560
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发表时间：
1992-07
影响因子：
11.1
通讯作者：
D. Walling;S. Edmiston;J. Sixbey;Mohamed Abdel-Hamid;Mohamed Abdel-Hamid;Lionel Resnick;N. Raab-Traub
中科院分区：
文献类型：
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作者：
D. Walling;S. Edmiston;J. Sixbey;Mohamed Abdel-Hamid;Mohamed Abdel-Hamid;Lionel Resnick;N. Raab-Traub
从毛状白斑标本中分析了爱泼斯坦-巴尔病毒DNA，毛状白斑是一种口腔病变，发生在感染人类免疫缺陷病毒的患者身上。Southern印迹分析和聚合酶链式反应证实同时存在1型和2型Epstein-Barr病毒。BamHI WYH区和EcoRI C区克隆的限制性片段长度多态提示存在多株1型和2型病毒。在Southern印迹分析和EBNA-2基因编码区的克隆上发现了多个大小可变的BamHI H片段，这也表明毛状白斑存在多个病毒株或变异株共感染。EBNA-2基因内部和周围的病毒基因组重组显然产生了有效复制的病毒变体，其中一个似乎随着时间的推移在病变中增加了丰度。这些数据表明，毛状白斑与多株复制的Epstein-Barr病毒和内源性病毒变异株共感染，其中一些病毒变异株具有EBNA-2基因突变。
Epstein-Barr virus DNA was analyzed from specimens of hairy leukoplakia, an oral lesion that occurs in patients infected with the human immunodeficiency virus. The simultaneous presence of both type 1 and type 2 Epstein-Barr virus was demonstrated by Southern blot analysis and polymerase chain reaction assay. Restriction fragment length polymorphisms in the BamHI WYH region and in clones of the EcoRI C region suggested the presence of multiple strains of type 1 and type 2 viruses. The demonstration of multiple variably sized BamHI H fragments on Southern blot analysis and cloning of the EBNA-2 gene coding region also suggested the presence of multiple viral strains or variants coinfecting hairy leukoplakia. Recombination of the viral genome in and around the EBNA-2 gene apparently generated viral variants that replicated efficiently, one of which appeared to increase in abundance in a lesion over time. These data indicate that hairy leukoplakia involves coinfection with multiple strains of replicating Epstein-Barr virus and the endogenous generation of viral variants, some of which have mutations of the EBNA-2 gene.