DEVELOPMENTAL REGULATION OF A GASTRULA-SPECIFIC GENE INJECTED INTO FERTILIZED XENOPUS EGGS
DEVELOPMENTAL REGULATION OF A GASTRULA-SPECIFIC GENE INJECTED INTO FERTILIZED XENOPUS EGGS
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DOI：
10.1002/j.1460-2075.1985.tb04105.x
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发表时间：
1985-01-01
期刊：
影响因子：
11.4
通讯作者：
MELTON, DA
中科院分区：
文献类型：
--
作者：
KRIEG, PA;MELTON, DA
为了研究非洲爪蟾早期发育过程中基因的转录调控，我们分离了一个在轻度囊胚转变时首先转录的基因。该基因（称为 GS17）的转录在妊娠中期停止，mRNA 迅速降解。因此，GS17 的转录本仅存在很短的一段时间，主要是在原肠胚形成期间。当 GS17 基因被注射到受精卵中时，注射 DNA 的转录会模仿内源基因的表达模式，即转录的开启和关闭都得到正确调节。注射的 DNA 并未显着扩增，仍处于染色体外。注入爪蟾卵的基因的正确表达将使研究参与激活胚胎基因组的母体因素成为可能。
To study the transcriptional regulation of genes during early Xenopus development, we have isolated a gene that is first transcribed at the mild-blastula transition. Transcription of this gene, called GS17, stops at mid-gestrula and the mRNA is rapidly degraded. Consequently, transcripts of GS17 are only present for a brief period, primarily during gastrulation. When the GS17 gene is injected into fertilized eggs, transcription from the injected DNA mimics the expression pattern of the endogneous gene, i.e., both the switch-on and switch-off of transcription are correctly regulated. The injected DNA is not significantly amplified and remains extrachromosomal. The correct expression of genes injected into Xenopus eggs wil make it possible to investigate maternal factors involved in activating the embryonic genome.