Voltammetric detection of superoxide production by photosystem II
Voltammetric detection of superoxide production by photosystem II
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DOI：
10.1016/s0014-5793(99)01067-4
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发表时间：
1999-09-03
期刊：
影响因子：
3.5
通讯作者：
Grace, SC
中科院分区：
文献类型：
--
作者：
Cleland, RE;Grace, SC
氧自由基在生物系统中扮演着病理和生理的双重角色，由于其暂时性和高效抗氧化机制的存在，此类自由基的检测是困难的。在植物中，氧的生理作用是双重的，氧是通过水的氧化产生的，并作为电子受体消耗。氧直接参与光合作用活动，使光合作用机构极有可能受到氧自由基的破坏。本文基于伏安法建立了一种直接、简便、快速的体外超氧化物歧化检测方法。它对其他研究超氧化物产生的体外系统具有潜在的应用价值。我们证明，除了光系统I产生超氧化物外，在还原条件下，光系统II也产生超氧化物，可能是从QA位产生。(C)1999年欧洲生化学会联合会。
Oxygen radicals play both pathological and physiological roles in biological systems, The detection of such radicals is difficult due to their transient nature and the presence of highly efficient antioxidant mechanisms. In plants the physiological role of oxygen is twofold, oxygen is produced by the oxidation of water and consumed as an electron acceptor. The direct involvement of oxygen in photosynthetic events exposes the photosynthetic apparatus to a high probability of damage by oxygen radicals. We report here a direct, simple and rapid method for the measurement of superoxide in vitro based on voltammetric detection. It has potential applications for other in vitro systems investigating superoxide production. We show that in addition to the well established production of superoxide from photosystem I, under reducing conditions superoxide is also produced by photosystem II, probably from the QA site. (C) 1999 Federation of European Biochemical Societies.