R-eriodictyol and S-eriodictyol exhibited comparable effect against H2O2-induced oxidative stress in EA.hy926 cells
R-eriodictyol and S-eriodictyol exhibited comparable effect against H2O2-induced oxidative stress in EA.hy926 cells
复制标题
R-圣草酚和 S-圣草酚在 EA.hy926 细胞中对 H2O2 诱导的氧化应激表现出类似的效果
DOI：
10.5582/ddt.2014.01033
复制
发表时间：
2014-10-01
影响因子：
3.1
通讯作者：
Ren, Dongmei
中科院分区：
文献类型：
--
作者：
Li, Haizhen;Li, Chao;Ren, Dongmei
圣草酚是一种黄烷酮，以其抗氧化活性而闻名。由于 C-2 位有手性碳原子，圣草酚始终以外消旋形式存在。为了研究每种对映体在H2O2诱导的氧化应激下的抗氧化活性，使用手性Amylose-C柱作为手性固定相，通过高效液相色谱（HPLC）分离圣草酚对映体。采用在线耦合HPLC-圆二色性(CD)法测定两种洗脱液的洗脱顺序和绝对构型。外消旋和对映体圣草酚对 H2O2 诱导的 EA 细胞毒性的保护作用。测试了hy926细胞。结果表明，圣草酚的两种对映体和相应的外消旋体是等价的，这表明C-2手性中心的构型不影响EA对H2O2诱导的氧化应激的细胞保护活性。 hy926细胞。
Eriodictyol is a flavanone well-known for its antioxidative activity. Due to a chiral carbon atom in position C-2, eriodictyol always exist in racemic form. In order to study the antioxidant activity under H2O2-induced oxidative stress of each enantiomer, enantiomers of eriodictyol were resolved by high-performance liquid chromatography (HPLC), using a Chiral Amylose-C column as chiral stationary phase. Online coupling HPLC-circular dichroism (CD) method was used for the determination of elution order and the absolute configurations of the two eluates. The protective effects of racemic and enantiomeric eriodictyol against H2O2-induced cytotoxicity with EA. hy926 cells were tested. The results showed that the two enantiomers of eriodictyol and the corresponding racemate were equipotent, suggesting that the configuration of the C-2 chiral center does not influence the cytoprotective activity against H2O2-induced oxidative stress in EA. hy926 cells.