Acute exercise increases nitric oxide synthase activity in skeletal muscle
Acute exercise increases nitric oxide synthase activity in skeletal muscle
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DOI：
10.1152/ajpendo.1999.277.2.e390
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发表时间：
1999-08-01
影响因子：
5.1
通讯作者：
Balon, TW
中科院分区：
文献类型：
--
作者：
Roberts, CK;Barnard, RJ;Balon, TW
本研究探讨急性运动对骨骼肌一氧化氮合酶（NOS）活性的影响。将雌性SD大鼠分为对照组、运动组和运动+N-硝基-L-精氨酸甲酯（L-NAME）组。在运动+ L-NAME组中，L-NAME在饮用水（1 mg/ml）中给药2天，随后运动和运动+ L-NAME组进行45分钟的力竭跑台运动，之后测量NOS活性和肌糖原。在对照组和运动组中，使用1-氨基-S-甲基异硫脲（AMITU）（一种选择性神经元NOS抑制剂），在有和没有额外的非选择性NOS阻断[用N-G-单甲基-L-精氨酸（L-NMMA）]的情况下，在体外评估nNOS对总NOS活性的贡献。与对照组相比，运动组的NOS活性增加了37%，两组的NOS活性均显著高于运动+ L-NAME组。AMITU使对照组和运动组的总NOS活性分别降低了31.8%和30.2%，并且这些活性在对照组和运动组中均显著大于AMITU + L-NMMA。我们的结论是：1）在骨骼肌中存在基础神经元NOS和内皮NOS活性，2）急性运动会增加骨骼肌中NOS活性，3）运动期间糖原消耗与NOS活性无关。
This study examined the effects of acute exercise on skeletal muscle nitric oxide synthase (NOS) activity. Female Sprague-Dawley rats were divided into three groups: control, exercise, and exercise +N-G-nitro-L-arginine methyl ester (L-NAME). In the exercise + L-NAME group, L-NAME was administered in the drinking water (1 mg/ml) for 2 days and subsequently the exercise and exercise + L-NAME groups underwent a 45-min bout of exhaustive treadmill running after which NOS activity and muscle glycogen were measured. In the control and exercise groups, 1-amino-S-methylisothiourea (AMITU), a selective neuronal NOS inhibitor, with and without additional nonselective NOS blockade [with N-G-monomethyl-L-arginine (L-NMMA)], was used in vitro to assess the contribution of nNOS to total NOS activity. The exercise bout increased NOS activity by 37% in exercise compared with control groups, and both groups had significantly greater NOS activity compared with exercise + L-NAME. AMITU decreased total NOS activity in the control and exercise groups by 31.8 and 30.2%, respectively, and these activities were significantly greater than AMITU + L-NMMA in both control and exercise groups. We conclude that 1) there is basal neuronal NOS and endothelial NOS activity in skeletal muscle, 2) an acute exercise bout increases NOS activity in skeletal muscle, and 3) glycogen depletion during exercise occurs irrespective of NOS activity.