Organization of the nervous system in the model planarian Schmidtea mediterranea:: An immunocytochemical study
Organization of the nervous system in the model planarian Schmidtea mediterranea:: An immunocytochemical study
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DOI：
10.1016/j.neures.2008.04.005
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发表时间：
2008-08-01
影响因子：
2.9
通讯作者：
Cebria, Francesc
中科院分区：
文献类型：
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作者：
Cebria, Francesc
淡水涡虫是一种在分子水平上研究再生的新兴模式。这些动物可以在短短几天内再生一个完整的中枢神经系统。近年来，在几个实验室使用的两种常见的涡虫物种中，已经确定了数百个神经系统表达的基因：Dugesia japonica和Schmidtea mediterranea。对其中一些神经基因的功能分析使这些动物的中枢神经系统再生过程得以阐明。然而，需要额外的工作来表征不同的神经元群。因此，鉴定或产生作为特定神经细胞类型标记的抗体不仅对获得更详细的涡虫神经系统特征，而且对通过RNA干扰获得的表型分析非常有用。在这里，我使用了五种不同的抗体来描述淡水涡虫地中海s的不同神经元群。这项研究代表了在细胞水平上表征该物种神经系统组织的第一步。(C) 2008爱思唯尔爱尔兰有限公司和日本神经科学学会。版权所有。
Freshwater planarians are an emerging model in which to study regeneration at the molecular level. These animals can regenerate a complete central nervous system (CNS) in only a few days. In recent years, hundreds of genes expressed in the nervous system have been identified in two popular planarian species used by several laboratories: Dugesia japonica and Schmidtea mediterranea. Functional analyses of some of those neural genes have allowed the process of CNS regeneration to begin to be elucidated in those animals. However, additional work is required to characterize the different neuronal populations. Thus, the identification or generation of antibodies that act as markers for specific neuronal cell types would be extremely useful not only in obtaining a more detailed characterization of the planarian nervous system but also for the analysis of phenotypes obtained by RNA interference. Here, I have used five different antibodies to describe different neuronal populations in the freshwater planarian S. mediterranea. This study represents a first step in characterizing the organization of the nervous system of this species at the cellular level. (C) 2008 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.