Exposure to bile and gastric juice can impact the aerodigestive microbiome in people with cystic fibrosis.
Exposure to bile and gastric juice can impact the aerodigestive microbiome in people with cystic fibrosis.
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暴露于胆汁和胃液可以影响囊性纤维化患者的呼吸消化微生物组。
DOI：
10.1038/s41598-022-15375-4
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发表时间：
2022-06-30
影响因子：
4.6
通讯作者：
Ward, Christopher
中科院分区：
文献类型：
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作者：
Al-Momani, Hafez;Perry, Audrey;Nelson, Andrew;Stewart, Christopher J;Jones, Rhys;Krishnan, Amaran;Robertson, Andrew;Bourke, Stephen;Doe, Simon;Cummings, Stephen;Anderson, Alan;Forrest, Tara;Forrest, Ian;Griffin, Michael;Wilcox, Matthew;Brodlie, Malcolm;Pearson, Jeffrey;Ward, Christopher
微生物群的研究揭示了肠道和肺部微生物之间的相互关系。这种关系可能会影响肺部疾病的进展，特别是在囊性纤维化（CF）患者中，他们经常经历食管外反流（EOR）。尽管确定了这种关系，但它并没有很好地描述。我们的假设是CF中的胃和肺微生物组相关，具有呼吸消化病理生理学的潜力。我们通过培养和16S rRNA基因测序对13例CF患者的胃和痰细菌群落进行了评估。评价了不同水平的胆汁酸、胃蛋白酶和pH对铜绿假单胞菌（Pa）患者分离株的影响。在有EOR症状的患者的胃和痰样本中鉴定了Pa和NTM的克隆相关菌株。细菌多样性更显着，相比，胃液中的大肠杆菌。胃和肺胆汁和胃蛋白酶水平与Pa生物膜形成相关。对具有阴性粪便的CF患者的呼吸消化微生物组的分析表明，肠道可以是Pa和NTM的储存库。这与CF患者的反流和潜在吸入症状相结合，突出了肠道和肺部微生物之间交流的可能性。这一现象值得进一步研究。
Studies of microbiota reveal inter-relationships between the microbiomes of the gut and lungs. This relationship may influence the progression of lung disease, particularly in patients with cystic fibrosis (CF), who often experience extraoesophageal reflux (EOR). Despite identifying this relationship, it is not well characterised. Our hypothesis is that the gastric and lung microbiomes in CF are related, with the potential for aerodigestive pathophysiology. We evaluated gastric and sputum bacterial communities by culture and 16S rRNA gene sequencing in 13 CF patients. Impacts of varying levels of bile acids, pepsin and pH on patient isolates of Pseudomonas aeruginosa (Pa) were evaluated. Clonally related strains of Pa and NTM were identified in gastric and sputum samples from patients with symptoms of EOR. Bacterial diversity was more pronounced in sputa compared to gastric juice. Gastric and lung bile and pepsin levels were associated with Pa biofilm formation. Analysis of the aerodigestive microbiomes of CF patients with negative sputa indicates that the gut can be a reservoir of Pa and NTM. This combined with the CF patient’s symptoms of reflux and potential aspiration, highlights the possibility of communication between microorganisms of the gut and the lungs. This phenomenon merits further research.