Fluorescence in situ hybridization mapping of human chromosome 19: cytogenetic band location of 540 cosmids and 70 genes or DNA markers.
Fluorescence in situ hybridization mapping of human chromosome 19: cytogenetic band location of 540 cosmids and 70 genes or DNA markers.
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人类 19 号染色体荧光原位杂交作图：540 个粘粒和 70 个基因或 DNA 标记的细胞遗传学条带位置。
DOI：
10.1006/geno.1993.1021
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发表时间：
1993
期刊：
影响因子：
4.4
通讯作者：
Carrano,A
中科院分区：
文献类型：
--
作者：
Trask,B;Fertitta,A;Christensen,M;Youngblom,J;Bergmann,A;Copeland,A;deJong,P;Mohrenweiser,H;Olsen,A;Carrano,A
我们在这里报告的带定位540 cosmetics映射到染色体19。通过荧光原位杂交（FISH）相对于DAPI/放线菌素染色产生的染色体条带绘制了Cosmetics。本文还详细分析了19号染色体上3种不同的亚端粒和22种近着丝粒区的cosplay在其它染色体上的分布。菌落杂交鉴定出142个含有代表基因或DNA标记的序列的染色体19。这些cosmetics的FISH映射sublocalizes共70个基因和19号染色体上的DNA标记，修改了以前发表的2个基因的地图分配，并缩小了超过20个标记的位置。
We report here the band location of 540 cosmids mapped to chromosome 19. The cosmids were mapped by fluorescencein situhybridization (FISH) relative to chromosomal bands produced by DAPI/actinomycin staining. The cosmids are distributed throughout the chromosome, with a sampling bias for the q-arm. A detailed analysis of the distribution of three different subtelomeric and 22 pericentromeric chromosome 19 cosmids on other chromosomes is also reported. Colony hybridization identified 142 cosmids that contain sequences representing genes or DNA markers that map to chromosome 19. FISH mapping of these cosmids sublocalizes a total of 70 genes and DNA markers on chromosome 19, revises the previously published map assignments of 2 genes, and narrows the location of over 20 markers.