Comparison of VP4 and VP7 of five murine rotavirus strains.
Comparison of VP4 and VP7 of five murine rotavirus strains.
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五种鼠轮状病毒株VP4和VP7的比较。
DOI：
10.1006/viro.1994.1482
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发表时间：
1994
期刊：
影响因子：
3.7
通讯作者：
Greenberg,HB
中科院分区：
文献类型：
--
作者：
Dunn,SJ;Burns,JW;Cross,TL;Vo,PT;Ward,RL;Bremont,M;Greenberg,HB
测定了五种鼠轮状病毒株（EW、EB、EC、EL、EHP）的外衣壳蛋白 VP4 和 VP7 的核苷酸和推导的氨基酸序列。将五种鼠轮状病毒株的VP7氨基酸序列与代表G血清型1-14的轮状病毒株的VP7氨基酸序列进行比较表明，鼠株彼此高度同源，并且与代表G3的株的相关性比与任何其他G型的株的相关性更密切。对小鼠品系的 VP4 氨基酸序列的分析揭示了至少两种小鼠 P 型的存在。因此，序列分析将预测鼠轮状病毒是G3或G3样病毒，并代表至少两种独特的P类型（暂定为EW、EB、EC的P17和EHP的EL和P18）。当我们试图通过血清中和试验对小鼠品系进行分类时，我们的结果不太清楚。通过使用几种原型 G3 菌株的聚焦还原中和测定，两种小鼠品系 EHP 和 EW 的血清显示出单向反应性。 G3 血清分型单克隆抗体 159 未能中和 EHP 和 EW，而 G3 特异性单克隆抗体 YO-1E2 中和 EHP，但不能中和 EW。含有来自 EW 的 VP7 和来自 RRV 的 VP4 的重配体 (A15) 被这两种 G3 特异性单克隆抗体中和，中和程度比 EW 高 8 至 20 倍，但仍比 SA11 和 RRV 低 64 至 250 倍。这些结果表明VP4可以影响VP7的抗原性。
The nucleotide and deduced amino acid sequences of outer capsid proteins VP4 and VP7 of five murine rotavirus strains (EW, EB, EC, EL, EHP) were determined. Comparisons of the VP7 amino acid sequences of the five murine rotavirus strains with rotavirus strains representative of G serotypes 1-14 showed that the murine strains were highly homologous to one another and more closely related to strains representing G3 than to any other G type. Analysis of the VP4 amino acid sequences of the murine strains revealed the presence of at least two murine P types. Therefore, sequence analysis would predict that the murine rotaviruses are G3 or G3-like and represent at least two unique P types (tentatively P17 for EW, EB, EC, and EL and P18 for EHP). When we attempted to categorize the murine strains by serum neutralization tests, our results were less clear. Serum to two murine strains, EHP and EW, displayed one-way reactivity by focus-reduction neutralization assays using several prototype G3 strains. The G3 serotyping monoclonal antibody 159 failed to neutralize EHP and EW, while the G3-specific monoclonal YO-1E2 neutralized EHP, but not EW. A reassortant (A15) containing VP7 from EW and VP4 from RRV was neutralized by these two G3-specific monoclonal antibodies to a revel 8- to 20-fold more than EW, but was still 64- to 250-fold less than either SA11 and RRV. These results suggest that VP4 can influence the antigenicity of VP7.