Adsorption of cyanuric chloride-activated polyethylene glycol on liposomes
Adsorption of cyanuric chloride-activated polyethylene glycol on liposomes
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DOI：
10.1016/s0927-7757(00)00845-1
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发表时间：
2001-06-30
影响因子：
5.2
通讯作者：
Estelrich, J
中科院分区：
文献类型：
--
作者：
Boada, J;Gallardo, M;Estelrich, J
一种获得空间稳定脂质体的方法包括将过量的氰尿酰氯活化的聚乙二醇5000（活化的PEG）与含有二硬脂酰磷脂酰乙醇胺的脂质体混合。对挤出的脂质体中存在的活化PEG的定量分析表明，可以发现其接枝以及涂覆在脂质体表面上。在恒定面积和表面活性下的渗透动力学已经显示了活化的PEG吸附在脂质体上的能力。这表明活化的PEG和脂质之间的相互作用主要是疏水性的，并且活化的PEG的三嗪基团必须嵌入脂质中。微电泳测量是一致的过程中观察到的吸附：PEG的掺入降低了相应的zeta电位，但PEG的存在下，在散装增加它们的绝对值。位于这两个极端之间的实验值表明PEG被吸附。(C)2001 Elsevier Science B.V.保留所有权利。
One method for obtaining sterically stabilized liposomes consists of mixing an excess of cyanuric chloride-activated polyethylene glycol 5000 (activated PEG) with liposomes bearing distearoylphosphatidylethanolamine. Quantitative analysis of activated PEG present in extruded liposomes suggested that it can be found grafted as well as coated on the liposomal surface. Penetration kinetics at constant area and surface activity have shown the ability of activated PEG to adsorb on liposomes. This suggests that the interaction between activated PEG and lipid is primarily hydrophobic, and the triazine group of activated PEG must be embedded in the lipid. Microelectrophoretic measurements are consistent with the process of adsorption observed: Incorporation of PEG reduces the corresponding zeta potentials, but the presence of PEG in the bulk increases them in absolute terms. An experimental value lying between such extremes indicates that PEG is adsorbed. (C) 2001 Elsevier Science B.V. All rights reserved.