Crucial role of the biological barrier at the primary targeted organs in controlling the translocation and toxicity of multi-walled carbon nanotubes in the nematode Caenorhabditis elegans
Crucial role of the biological barrier at the primary targeted organs in controlling the translocation and toxicity of multi-walled carbon nanotubes in the nematode Caenorhabditis elegans
复制标题
主要靶器官的生物屏障在控制线虫秀丽隐杆线虫中多壁碳纳米管的易位和毒性中的关键作用
DOI：
10.1039/c3nr03917j
复制
发表时间：
2013-01-01
期刊：
影响因子：
6.7
通讯作者：
Wang, Dayong
中科院分区：
文献类型：
--
作者：
Wu, Qiuli;Li, Yinxia;Wang, Dayong
多壁碳纳米管(MWCNTs)可以被转移到生物体的靶器官中。我们利用线虫秀丽线虫的模式生物来研究初级靶器官的生物屏障在调节多壁碳纳米管的移位和毒性形成中的作用。在预测的环境相关浓度下，长期暴露于多壁碳纳米管会对线虫产生与初级和次级靶器官相关的不良影响。聚乙二醇化修饰在降低多壁碳纳米管毒性中的作用可能主要是通过抑制其通过初级靶向器官向次级靶器官的移位所致。初级靶器官上的生物屏障对控制多壁碳纳米管向次级靶器官的移位起到了很大作用，这一点从初级靶器官中防止氧化应激所需的锰超氧化物歧化酶的功能中可见一斑。MN-SODS在主要靶器官的过表达有效地抑制了MWCNTs的易位和毒性。我们的工作强调了主要靶器官的生物屏障在调节多壁碳纳米管的移位和毒性形成方面的关键作用。我们的数据还为改善生物相容性的工程纳米材料(ENM)的未来发展以及针对ENMS毒性的预防策略的设计提供了线索。
Multi-walled carbon nanotubes (MWCNTs) can be translocated into the targeted organs of organisms. We employed a model organism of the nematode Caenorhabditis elegans to investigate the role of a biological barrier at the primary targeted organs in regulating the translocation and toxicity formation of MWCNTs. A prolonged exposure to MWCNTs at predicted environmental relevant concentrations caused adverse effects associated with both the primary and secondary targeted organs on nematodes. The function of PEGylated modification in reducing MWCNTs toxicity might be mainly due to the suppression of their translocation into secondary targeted organs through the primary targeted organs. A biological barrier at the primary targeted organs contributed greatly to the control of MWCNTs translocation into secondary targeted organs, as indicated by functions of Mn-SODs required for prevention of oxidative stress in the primary targeted organs. Over-expression of Mn-SODs in primary targeted organs effectively suppressed the translocation and toxicity of MWCNTs. Our work highlights the crucial role of the biological barrier at the primary targeted organs in regulating the translocation and toxicity formation of MWCNTs. Our data also shed light on the future development of engineered nanomaterials (ENMs) with improved biocompatibility and design of prevention strategies against ENMs toxicity.