Exopolysaccharides from Lactobacillus plantarum induce biochemical and physiological alterations in tomato plant against bacterial spot
Exopolysaccharides from Lactobacillus plantarum induce biochemical and physiological alterations in tomato plant against bacterial spot
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植物乳杆菌胞外多糖诱导番茄植株发生细菌性斑点病的生化和生理变化
DOI：
10.1007/s00253-018-8946-0
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发表时间：
2018
影响因子：
5
通讯作者：
Robson Marcelo Di Piero
中科院分区：
文献类型：
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作者：
Juliane Mendes Lemos Blainski;Argus Cesar Rocha Neto;E. Schimidt;J. Voltolini;M. Rossi;Robson Marcelo Di Piero
本研究旨在探讨植物乳杆菌产生的胞外多糖（EPS）控制细菌斑疹的有效性和引发防御机制，并验证番茄植株生理反应的变化。5片终叶的番茄植株分别喷洒EPS、酸性苯并甲醚（ASM，阳性对照）或蒸馏水（阴性对照），3天后接种栀子黄单胞菌。接种后7、15和21天评估局部和全身保护。定量了生化防御机制（过氧化物酶[POX]、多酚氧化酶[PPO]、过氧化氢酶[CAT]、超氧化物歧化酶[SOD]、过氧化氢[H2O2]积累和生理变化）。此外，通过光镜和荧光显微镜对接种或未接种加特纳氏杆菌的番茄植株进行了组织化学分析。平均而言，与对照植物相比，EPS和ASM分别使处理叶片的细菌病严重程度降低了55%和96%。经eps处理的植株受到病原菌侵袭后，PPO、CAT和SOD的活性均有所增加。ASM引起所有酶的升高，特别是在接种植株中。与接种对照植株相比，喷施EPS后植株的光合速率提高了3倍，气孔导度和蒸腾速率降低了36%。光化学效率和土壤植物分析发育（SPAD）指数没有变化。在光镜和荧光显微镜下，可以看到EPS处理的叶片表皮中纤维素化合物的积累。因此，应用EPS可作为防治番茄细菌性斑疹病的一种替代方法。该研究探讨了植物中多糖引起的生化和生理变化如何有助于降低疾病的严重程度。
This study aimed to evaluate the effectiveness of exopolysaccharides (EPS) produced byLactobacillus plantarumin controlling bacterial spot and eliciting defense mechanisms, and verify alterations in the tomato plant physiological responses. Tomato plants with five definitive leaves were sprayed with EPS, acibenzolar-S-methyl (ASM, positive control), or distilled water (negative control) and inoculated withXanthomonas gardneriafter 3 days. Local and systemic protection was evaluated 7, 15, and 21 days after inoculation. Biochemical defense mechanisms (peroxidases [POX], polyphenoloxidases [PPO], catalase [CAT], superoxide dismutase [SOD], accumulation of hydrogen peroxide [H2O2], and physiological changes) were quantified. In addition, histochemical analyses were examined through light and fluorescence microscopy in treated tomato plants, inoculated or not withX.gardneri. On average, EPS and ASM provided 55 and 96% reduction, respectively, of the bacteriosis severity in treated leaves, compared to the control plants. Increased activities of PPO, CAT, and SOD were found in the EPS-treated plants after being challenged with the pathogen. ASM caused increases in all enzymes analyzed, especially in inoculated plants. The rate of photosynthesis was three times higher, while stomatal conductance and transpiration were 36% lower in the plants sprayed with EPS and challenged with the pathogen, compared to the inoculated control plants. The photochemical efficiency and Soil Plant Analysis Development (SPAD) index did not change. Under light and fluorescence microscopy, it could be seen accumulation of cellulosic compounds in the epidermis of the leaves treated with EPS. Therefore, application of EPS can be considered an alternative for the control of bacterial spot in tomato plants. The study discusses how biochemical and physiological alterations caused by the polysaccharide in the plants contributed to reduce the severity of the disease.