Electrophysiological and morphological characterization of rat embryonic motoneurons in a defined system
Electrophysiological and morphological characterization of rat embryonic motoneurons in a defined system
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DOI：
10.1021/bp034076l
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发表时间：
2003-11-01
影响因子：
2.9
通讯作者：
Hickman, JJ
中科院分区：
文献类型：
--
作者：
Das, M;Molnar, P;Hickman, JJ
为了将生物组分与硅基器件和系统相结合，人工硅烷表面已成功用于在特定环境中培养运动神经元。在这项研究中，我们对在N - 1[3 -（三甲氧基甲硅烷基）丙基]二亚乙基三胺（DETA）自组装单分子层（SAM）上以及在无血清培养基中的鸟氨酸/层粘连蛋白表面上生长的纯化大鼠胚胎（E14）运动神经元的形态和电生理学进行了表征。在DETA上，运动神经元呈扁平状且长出更多的突起，而在鸟氨酸/层粘连蛋白上它们往往会聚集。膜时间常数是与电紧张紧密性相关的一个特性，在DETA上生长的运动神经元的膜时间常数明显更长。对于在不同表面上的运动神经元，其他电生理参数相似。这是第一项将纯化的腹角运动神经元在完全确定的（非生物表面，无血清）环境中培养的研究。
In an attempt to integrate biological components with silicon-based devices and systems, artificial silane surfaces have been successfully used to grow motoneurons in a defined environment. In this study we characterized the morphology and electrophysiology of purified rat embryonic (E14) motoneurons grown on a self-assembled monolayer (SAM) of N-1[3-(trimethoxysilyl)propyl] diethylenetriamine (DETA) versus that on ornithine/laminin surfaces in serum-free media. On DETA motoneurons were flat and grew more processes, whereas on ornithine/laminin they tended to aggregate. The membrane time constant, a characteristic associated with electrotonic compactness, was significantly longer for motoneurons grown on DETA. Other electrophysiological parameters were similar for the motoneurons on the different surfaces. This is the first study where purified ventral horn motoneurons were cultured in a completely defined (nonbiological surface, serum-free) environment.