Identification of the hemoglobin scavenger receptor/CD 163 as a natural soluble protein in plasma
Identification of the hemoglobin scavenger receptor/CD 163 as a natural soluble protein in plasma
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DOI：
10.1182/blood.v99.1.378
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发表时间：
2002-01-01
期刊：
影响因子：
20.3
通讯作者：
Moestrup, SK
中科院分区：
文献类型：
--
作者：
Moller, HJ;Peterslund, NA;Moestrup, SK
血红蛋白清道夫受体（HbSR/CD 163）是一种白细胞介素-6和糖皮质激素调节的巨噬细胞/单核细胞受体，用于摄取触珠蛋白-血红蛋白复合物。此外，有强烈的迹象表明HbSR具有抗炎功能。免疫沉淀和免疫印迹能够鉴定具有与由胞外结构域（清道夫受体富含半胱氨酸1-9）组成的重组HbSR的电泳迁移率相等的电泳迁移率的HbSR的可溶性血浆形式（sHbSR）。建立了夹心酶联免疫吸附试验，并用于测量130名健康受试者的sHbSR水平（中位数，1.87 mg/L;范围，0.73-4.69 mg/L）。为了评估白细胞刺激和增殖增加的情况下的sHbSR水平，对血液科收治的140名患者进行了筛选。几个患者，诊断范围广，sHbSR水平高于健康人的范围。粒单核细胞白血病和肺炎/脓毒症患者的水平最高（高达67.3 mg/L）。结论：sHbSR是一种丰富的血浆蛋白，在监测感染和粒单核细胞白血病患者中具有潜在价值。（血。2002;99：378-380）（C）2002年由美国血液学会。
The hemoglobin scavenger receptor (HbSR/CD163) is an interleukin-6- and glucocorticoid-regulated macrophage/ monocyte receptor for uptake of haptoglobin-hemoglobin complexes. Moreover, there are strong indications that HbSR serves an anti-inflammatory function. Immunoprecipitation and immunoblotting enabled identification of a soluble plasma form of HbSR (sHbSR) having an electrophoretic mobility equal to that of recombinant HbSR consisting of the extracellular domain (scavenger receptor cysteine-rich 1-9). A sandwich enzyme-linked immunosorbent assay was established and used to measure the sHbSR level in 130 healthy subjects (median, 1.87 mg/L; range, 0.73-4.69 mg/L). To evaluate the sHbSR levels in conditions with increased leukocyte stimulation and proliferation, 140 patients admitted to a hematological department were screened. Several patients, with a broad spectrum of diagnoses, had a level of sHbSR above the range of healthy persons. Patients with myelomonocytic leukemias and pneumonia/sepsis exhibited the highest levels (up to 67.3 mg/L). In conclusion, sHbSR is an abundant plasma protein potentially valuable In monitoring patients with Infections and myelomonocytic leukemia. (Blood. 2002;99:378-380) (C) 2002 by The American Society of Hematology.