Methacrylate-ester-based Reversed Phase Monolithic Columns for High Speed Separation Prepared by Low Temperature UV Photo-polymerization
Methacrylate-ester-based Reversed Phase Monolithic Columns for High Speed Separation Prepared by Low Temperature UV Photo-polymerization
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DOI：
10.2116/analsci.25.1107
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发表时间：
2009-09-01
影响因子：
1.6
通讯作者：
Ohtani, Hajime
中科院分区：
文献类型：
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作者：
Hirano, Tomohiko;Kitagawa, Shinya;Ohtani, Hajime
采用紫外光聚合法制备了甲基丙烯酸丁酯反相毛细管整体柱。考察了两种光聚合条件(紫外光照射强度和聚合温度)对柱性能的影响。较高的紫外光照射强度和较低的聚合温度均可获得较好的柱效。在优化的条件下制备的色谱柱在1~110 mm/S的线性流速范围内对尿嘧啶和5种烷基苯类化合物进行了分离，在1 mm/S的流速下，所有分析物都得到了很好的分离(N=36000~45000板/m)。在8 S内的高速分离是在室温下以110 mm/S(背压，33 Mpa)进行的，而先前洗脱的峰部分重叠。流速与背压的关系表明，在50-110 mm/S范围内，整体柱可能会发生某种结构变化，但测量后没有观察到明显的或滞后的变化。
Butyl methacrylate-based reversed phase capillary monolithic columns were prepared using ultraviolet (UV) photo-polymerization. The effects of two photo-polymerization conditions (UV irradiation intensity and polymerization temperature) on the column characteristics were investigated. Both the higher UV irradiation intensity and the lower polymerization temperature lead to the superior column efficiency. The column prepared under the optimized conditions was evaluated through the separation of the uracil and five alkylbenzenes in the linear flow rate range of 1 - 110 mm/s. At 1 mm/s, all analytes were well separated (N = 36000 - 45000 plates/m). The high speed separation within 8 s was performed at 110 mm/s (back pressure, 33 MPa) at room temperature, whereas the peaks eluted earlier were overlapped partially. The relationship between the flow rate and the back pressure indicated that some kind of structural change of the monolith might occur in 50 - 110 mm/s, although no visible or hysteresis changes of the monolith were observed after the measurement.