A functional genomics approach using metabolomics and in silico pathway analysis
A functional genomics approach using metabolomics and in silico pathway analysis
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DOI：
10.1002/bit.10378
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发表时间：
2002-09-30
影响因子：
3.8
通讯作者：
Nielsen, J
中科院分区：
文献类型：
--
作者：
Förster, J;Gombert, AK;Nielsen, J
在功能基因组学领域，越来越多的努力正在进行使用代谢组数据分析孤儿基因的功能。改进的分析设备可以同时筛选大量的代谢物。使用多变量数据分析技术分析此类代谢物谱，并且基因型的变化在许多情况下将导致不同的代谢物谱。在这里，一个理论框架，可应用于确定孤儿基因的功能。该方法基于代谢组学分析与计算机途径分析的组合。途径分析可以使用凸分析进行，并且在不同代谢物谱中表达的缺失突变体的活性途径结构的变化可以揭示孤儿基因的功能或功能类别。这个概念是用一个简化的模型来说明酿酒酵母的生长。(C)2002 Wiley Periodicals，Inc.
In the field of functional genomics increasing effort is being undertaken to analyze the function of orphan genes using metabolome data. Improved analytical equipment allows screening simultaneously for a high number of metabolites. Such metabolite profiles are analyzed using multivariate data analysis techniques and changes in the genotype will in many cases lead to different metabolite profiles. Here, a theoretical framework that may be applied to identify the function of orphan genes is presented. The approach is based on a combination of metabolome analysis combined with in silico pathway analysis. Pathway analysis may be carried out using convex analysis and a change in the active pathway structure of deletion mutants expressed in a different metabolite profile may disclose the function or the functional class of an orphan gene. The concept is illustrated using a simplified model for growth of Saccharomyces cerevisiae. (C) 2002 Wiley Periodicals, Inc.