QTL Mapping of Enzymatic Saccharification in Short Rotation Coppice Willow and Its Independence from Biomass Yield
QTL Mapping of Enzymatic Saccharification in Short Rotation Coppice Willow and Its Independence from Biomass Yield
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DOI：
10.1007/s12155-010-9077-3
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发表时间：
2010-09-01
影响因子：
3.6
通讯作者：
Murphy, Richard J.
中科院分区：
文献类型：
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作者：
Brereton, Nicholas J. B.;Pitre, Frederic E.;Murphy, Richard J.
短轮伐期矮林柳树（Salix spp.）是快速生长的木本植物，可以在较短的生长周期内以较低的农业化学品投入实现高生物量产量。SRC杨柳的生物质已经用于供热和发电，但其作为液体运输生物燃料的木质纤维素来源的潜力仍有待评估。在从木质纤维素生产生物乙醇中，酶促糖化被用作从植物细胞壁中的纤维素释放葡萄糖的方法。本研究以杨柳作图群体的138个基因型为材料，分析了柳树茎生物量酶解葡萄糖的变异，研究了该性状与生物量产量性状之间的关系，并确定了与酶解糖化产量相关的数量性状位点（QTL）。从杨柳茎生物量的葡萄糖产量中发现了显著的自然变化。该性状与生物产量性状无关。四个酶衍生的葡萄糖QTL被定位到染色体V，X，XI和XVI，表明酶糖化产量受到显着的遗传影响。我们的研究结果表明，SRC杨柳作为生物乙醇的来源具有很强的潜力，并可能有机会改善柳树的育种计划，以提高酶糖化产量和生物燃料生产。
Short rotation coppice (SRC) willows (Salix spp.) are fast-growing woody plants which can achieve high biomass yields over short growth cycles with low agrochemical inputs. Biomass from SRC willow is already used for heat and power, but its potential as a source of lignocellulose for liquid transport biofuels has still to be assessed. In bioethanol production from lignocellulose, enzymatic saccharification is used as an approach to release glucose from cellulose in the plant cell walls. In this study, 138 genotypes of a willow mapping population were used to examine variation in enzymatic glucose release from stem biomass to study relationships between this trait and biomass yield traits and to identify quantitative trait loci (QTL) associated with enzymatic saccharification yield. Significant natural variation was found in glucose yields from willow stem biomass. This trait was independent of biomass yield traits. Four enzyme-derived glucose QTL were mapped onto chromosomes V, X, XI, and XVI, indicating that enzymatic saccharification yields are under significant genetic influence. Our results show that SRC willow has strong potential as a source of bioethanol and that there may be opportunities to improve the breeding programs for willows for increasing enzymatic saccharification yields and biofuel production.