Relapse cell population differs from acute onset clone as shown by absence of the initially activated N-ras oncogene in a patient with acute myelomonocytic leukemia.
Relapse cell population differs from acute onset clone as shown by absence of the initially activated N-ras oncogene in a patient with acute myelomonocytic leukemia.
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复发细胞群与急性发病克隆不同，急性粒单核细胞白血病患者中不存在最初激活的 N-ras 癌基因。
DOI：
10.1182/blood.v72.3.931.bloodjournal723931
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发表时间：
1988
期刊：
影响因子：
20.3
通讯作者：
C. Moroni
中科院分区：
文献类型：
--
作者：
H. Senn;J. Jiricny;M. Fopp;L. Schmid;C. Moroni
我们用聚合酶链反应方法和合成的寡核苷酸杂交探针对1例N-ras突变（Gln61-Lys61）的髓单核细胞白血病患者进行了随访研究。这种方法使我们能够在群体中检测到低至3%的N-ras突变细胞。当患者进入临床缓解期时，突变变得无法检测。当复发发生时，原始细胞不携带N-ras突变。对复发DNA中M13克隆扩增的N-ras序列的分析仅显示N-ras基因的野生型等位基因。这些发现表明复发细胞群体来源于与急性期群体不同的克隆。此外，数据表明，N-ras突变不是急性粒单核细胞白血病（AMML）的起始病变。
We have conducted a follow-up study of a patient with myelomonocytic leukemia exhibiting an N-ras mutation (Gln61----Lys61) using the polymerase chain reaction method and synthetic oligonucleotide hybridization probes. This method allowed us to detect as little as 3% of N-ras-mutated cells within a population. When the patient went into clinical remission, the mutation became undetectable. When a relapse occurred, the blasts did not carry the N-ras mutation. Analysis of M13 cloned amplified N-ras sequences from relapse DNA revealed exclusively the wild type allele of the N-ras gene. These findings suggest that the relapse cell population is derived from a different clone than the acute phase population. Furthermore, the data argue that N-ras mutation is not an initiating lesion in this case of acute myelomonocytic leukemia (AMML).
DOI：
10.1073/pnas.83.24.9418
发表时间：
1986-12-01
影响因子：
11.1
作者：
MCMAHON, G;HANSON, L;WOGAN, GN
通讯作者：
WOGAN, GN
正常和突变 ras 蛋白在人类急性白血病中的表达。
影响因子：
8
作者：
Shen,WP;Aldrich,TH;Venta-Perez,G;FranzaJr,BR;Furth,ME
通讯作者：
Furth,ME