Evaluation of the antioxidant activity of enzymatically-hydrolyzed sulfated polysaccharides extracted from red algae; Pterocladia capillacea
Evaluation of the antioxidant activity of enzymatically-hydrolyzed sulfated polysaccharides extracted from red algae; Pterocladia capillacea
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DOI：
10.1016/j.lwt.2015.04.024
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发表时间：
2015-10-01
影响因子：
6
通讯作者：
Rifaat, Dalia
中科院分区：
文献类型：
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作者：
Fleita, Daisy;El-Sayed, Mayyada;Rifaat, Dalia
从红藻Pterocladia capillacea中提取硫酸多糖，并采用不同的酶对硫酸多糖进行水解。测定了提取物及其水解产物对DPPH自由基和过氧化氢的清除活性以及酚类物质的含量。酶解液中酚类物质含量最高，DPPH自由基清除率达92%，比酶解液提高50%以上。它还显示出相对于商业抗生素的高抗菌活性。酚类物质含量测定和HPLC分析表明，粘酶酶解物的高抗氧化活性可能与其短链结构、高含量的酚类物质以及多糖链中甘露糖和半乳糖的优势有关。(C)2015爱思唯尔有限公司版权所有。
Sulfated polysaccharides (SPs) were extracted from red algae, Pterocladia capillacea and the produced fractions were hydrolyzed using different enzymes. 1,1-Diphenyl-2-Picryl-Hydrazyl (DPPH) and peroxide scavenging activities as well as phenolic content of the extracted fractions and their hydrolysates were determined. Viscozyme hydrolysate exhibited the highest phenolic content and a DPPH scavenging activity of about 92% with more than 50% increase over its mother fraction. It also showed a high antibacterial activity relative to commercial antibiotics. Phenolic content determination and HPLC analysis suggested that the high antioxidant activity of viscozyme hydrolysate could be owed to its short-length chain and its high content of phenolic compounds as well as the predominance of mannose and galactose sugars in the polysaccharide chain. (C) 2015 Elsevier Ltd. All rights reserved.