In vitro characterization of vascular endothelial growth factor and dexamethasone releasing hydrogels for implantable probe coatings
In vitro characterization of vascular endothelial growth factor and dexamethasone releasing hydrogels for implantable probe coatings
复制标题
DOI：
10.1016/j.biomaterials.2004.07.069
复制
发表时间：
2005-06-01
期刊：
影响因子：
14
通讯作者：
Reichert, WM
中科院分区：
文献类型：
--
作者：
Norton, LW;Tegnell, E;Reichert, WM
为了提高生物传感器的生物相容性，研究了由甲基丙烯酸羟乙酯、1-乙烯-2-吡咯烷酮和聚乙二醇制得的防污水凝胶涂层。将这些涂层修饰成聚丙交酯-乙交酯共聚物(PLGA)微球，以释放地塞米松(DX)和/或血管内皮生长因子(VEGF)。在2周和1个月的研究中，测定了微球、水凝胶和包埋在水凝胶中的微球的DX和VEGF的释放动力学。总体而言，整体的、不可降解的水凝胶药物释放最初有一次爆裂，随后释放的量明显较低。微球药物释放动力学表现为先爆裂后缓释1个月。将微球包埋在水凝胶中会导致药物传递的减弱。与水凝胶释放的197+/-33 ng相比，包埋微球释放的血管内皮生长因子仅为1.1+/-0.3 ng，微不足道。在DX水凝胶首次破裂后，DX从包埋微球中的释放与水凝胶相似。水凝胶中DX的总释放量为155+/-35µg，高于包埋微球的60+/-6µg。根据这项研究，应该在水凝胶中加入血管内皮生长因子和DX，或者在水凝胶中或在水凝胶中嵌入DX微球，来制备水凝胶传感器涂层。(C)2004爱思唯尔有限公司。保留所有权利。
Anti-fouling hydrogel coatings, copolymers of 2-hydroxyethyl methacrylate, 1-vinyl-2-pyrrolidinone, and polyethylene glycol, were investigated for the purpose of improving biosensor biocompatibility. These coatings were modified to incorporate poly(lactide-co-glycolide) (PLGA) microspheres in order to release dexamethasone (DX) and/or vascular endothelial growth factor (VEGF). DX and VEGF release kinetics from microspheres, hydrogels, and microspheres embedded in hydrogels were determined in 2-week and 1-month studies. Overall, monolithic, non-degradable hydrogel drug release had an initial burst followed by release at a significantly lower amount. Microsphere drug release kinetics exhibited an initial burst followed by sustained release for 1 month. Embedding microspheres in hydrogels resulted in attenuated drug delivery. VEGF release from embedded microspheres, 1.1 +/- 0.3 ng, was negligible compared to release from hydrogels, 197 +/- 33 ng. After the initial burst from DX-loaded hydrogels, DX release from embedded microspheres was similar to that of hydrogels. The total DX release from hydrogels, 155 +/- 35 mug, was greater than that of embedded microspheres, 60 +/- 6 mug. From this study, hydrogel sensor coatings should be prepared incorporating VEGF in the hydrogel and DX either in the hydrogel or in DX microspheres embedded in the hydrogel. (C) 2004 Elsevier Ltd. All rights reserved.