Gold/alpha-lactalbumin nanoprobes for the imaging and treatment of breast cancer.
Gold/alpha-lactalbumin nanoprobes for the imaging and treatment of breast cancer.
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DOI：
10.1038/s41551-020-0584-z
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发表时间：
2020-07
影响因子：
28.1
通讯作者：
Kircher MF
中科院分区：
文献类型：
--
作者：
Yang J;Wang T;Zhao L;Rajasekhar VK;Joshi S;Andreou C;Pal S;Hsu HT;Zhang H;Cohen IJ;Huang R;Hendrickson RC;Miele MM;Pei W;Brendel MB;Healey JH;Chiosis G;Kircher MF
理想情况下，治疗药物应该是完全清除和可生物降解的。本文报道了乳金属蛋白-乳蛋白稳定的荧光超小金量子团簇的合成、表征和治疗应用。我们合成了三种类型的纳米探针，当通过光学彩色编码在单一波长激发时，它们一起显示可见光和近红外光谱上的荧光。在活体荷瘤小鼠中，近红外纳米探针产生荧光、x射线计算机断层扫描和磁共振成像的对比，并表现出循环时间长、在网状内皮系统中蓄积少、持续的肿瘤保留、轻微的毒性和肾脏清除。一种具有大斯托克斯位移的静脉注射近红外纳米探针促进了使用改进光学的智能手机在体内检测和图像引导切除乳腺肿瘤。此外，纳米探针中α -乳清蛋白部分展开的结构有助于与油酸形成抗癌脂蛋白复合物，从而抑制MAPK和PI3K-AKT通路，导致免疫原性细胞死亡和浸润性巨噬细胞的募集。纳米探针的可生物降解性和安全性使其适合于癌症的全身检测和局部治疗。
Theranostic agents should ideally be renally cleared and biodegradable. Here, we report the synthesis, characterization and theranostic applications of fluorescent ultrasmall gold quantum clusters that are stabilized by the milk metalloprotein alpha-lactalbumin. We synthesized three types of these nanoprobes that together display fluorescence across the visible and near-infrared spectra when excited at a single wavelength through optical colour coding. In live tumour-bearing mice, the near-infrared nanoprobe generates contrast for fluorescence, X-ray computed tomography and magnetic resonance imaging, and exhibits long circulation times, low accumulation in the reticuloendothelial system, sustained tumour retention, insignificant toxicity and renal clearance. An intravenously administrated near-infrared nanoprobe with a large Stokes shift facilitated the detection and image-guided resection of breast tumours in vivo using a smartphone with modified optics. Moreover, the partially unfolded structure of alpha-lactalbumin in the nanoprobe helps with the formation of an anti-cancer lipoprotein complex with oleic acid that triggers the inhibition of the MAPK and PI3K–AKT pathways, immunogenic cell death and the recruitment of infiltrating macrophages. The biodegradability and safety profile of the nanoprobes make them suitable for the systemic detection and localized treatment of cancer.
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影响因子：
11.2
作者：
Chen L;Mayer JA;Krisko TI;Speers CW;Wang T;Hilsenbeck SG;Brown PH
通讯作者：
Brown PH
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
8
作者：
Bhat AA;Pope JL;Smith JJ;Ahmad R;Chen X;Washington MK;Beauchamp RD;Singh AB;Dhawan P
通讯作者：
Dhawan P
影响因子：
11.2
作者：
Deckers, M;van Dinther, M;ten Dijke, P
通讯作者：
ten Dijke, P
DJ-1 通过阻止 pro-caspase-8 募集到 FADD 来抑制 TRAIL 诱导的细胞凋亡
影响因子：
8
作者：
Fu, K.;Ren, H.;Wang, G.
通讯作者：
Wang, G.