Spontaneous lytic replication and epitheliotropism define an Epstein-Barr virus strain found in carcinomas.
Spontaneous lytic replication and epitheliotropism define an Epstein-Barr virus strain found in carcinomas.
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DOI：
10.1016/j.celrep.2013.09.012
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发表时间：
2013-10
期刊：
影响因子：
8.8
通讯作者：
M. Tsai;A. Raykova;O. Klinke;Katharina Bernhardt;Kathrin Gärtner;C. Leung;K. Geletneky;S. Sertel
中科院分区：
文献类型：
--
作者：
M. Tsai;A. Raykova;O. Klinke;Katharina Bernhardt;Kathrin Gärtner;C. Leung;K. Geletneky;S. Sertel
爱泼斯坦-巴尔病毒（EBV）存在于多种肿瘤中，其发病率在世界各地差异很大。一个未被充分探索的假设是，特定的EBV菌株解释了这种现象。我们报道从中国鼻咽癌（NPC）患者中分离的病毒M81与其他鼻咽癌病毒有显著的相似性，但与所有其他已知的病毒株不同。与普通菌株相比，M81表现出相反的倾向性，感染B细胞的能力降低，感染上皮细胞的倾向高，这与它从肿瘤中分离出来的结果一致。M81在体外和体内的B细胞中自发复制的水平异常高，这与在鼻咽癌患者中观察到的病毒复制增强一致。自发复制和上皮性可部分归因于病毒蛋白内的多态性。我们建议将M81及其密切相关的分离株视为具有增强致病性的EBV亚型。
The Epstein-Barr virus (EBV) is found in a variety of tumors whose incidence greatly varies around the world. A poorly explored hypothesis is that particular EBV strains account for this phenomenon. We report that M81, a virus isolated from a Chinese patient with nasopharyngeal carcinoma (NPC), shows remarkable similarity to other NPC viruses but is divergent from all other known strains. M81 exhibited a reversed tropism relative to common strains with a reduced ability to infect B cells and a high propensity to infect epithelial cells, which is in agreement with its isolation from carcinomas. M81 spontaneously replicated in B cells in vitro and in vivo at unusually high levels, in line with the enhanced viral replication observed in NPC patients. Spontaneous replication and epitheliotropism could be partly ascribed to polymorphisms within viral proteins. We suggest considering M81 and its closely related isolates as an EBV subtype with enhanced pathogenic potential.