Immunophenotypic analysis of anaplastic large cell lymphoma by flow cytometry
Immunophenotypic analysis of anaplastic large cell lymphoma by flow cytometry
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DOI：
10.1309/hefl7kc435lfwex8
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发表时间：
2003-02-01
影响因子：
3.5
通讯作者：
Li, SY
中科院分区：
文献类型：
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作者：
Juco, J;Holden, JT;Li, SY
应用多参数流式细胞术对19例间变性大细胞淋巴瘤(ALCL)的抗原表达谱进行了研究。所有病例的肿瘤细胞均表达CD45、HLA-DR和CD30。每例患者至少表达1个T细胞相关抗原(CD2 12117[71%]；CD4 12119[63%]；CD3 6119[32%]；CD7 6119[32%]；CD5 5119[26%]；CD8 4119[21%])。CD25阳性表达14例(88%)。17例中有8例(47%)意外表达CD13。1例CD13+ALCL表达CD33，2例表达CD15。CD19、CD20、CD22、CD14、CD36不表达。通过流式细胞仪免疫表型分析(M)，在约33%(319)的ALCL中检测到间变性淋巴瘤激酶蛋白；免疫组织化学分析证实了其表达。在19例ALCL中，12例仅根据FCI结果结合体液(1例)、细针抽吸(3例)或切除活检标本(8例)的形态学检查而诊断。其余7例经FCI明确诊断，并经免疫组织化学分析证实。FCI有助于ALCL的诊断，特别是与细针抽吸检查相结合。髓系抗原异常表达的ALCL不应误诊为髓外髓系肿瘤。
We studied the antigen expression profiles of 19 anaplastic large cell lymphoma (ALCL) cases by multiparameter flow cytometry. The neoplastic cells expressed CD45, HLA-DR, and CD30 in all cases. At least 1 T cell-associated antigen was expressed in each case (CD2, 12117 [71 %]; CD4, 12119 [63 %]; CD3, 6119 [32%]; CD7, 6119 [32%]; CD5, 5119 [26%]; CD8, 4119 [21 %]). CD25 was expressed in 14 (88%) of 16 cases. CD13 was expressed unexpectedly in 8 (47%) of 17 cases. One CD13+ ALCL also was positive for CD33 and 2 others for CD15. CD19, CD20, CD22, CD14, and CD36 were not expressed. Anaplastic lymphoma kinase protein was detected in about 33% (319) of ALCLs examined by flow cytometric immunophenotyping (M); expression was validated by immunohistochemical analysis. Of 19 ALCL cases, 12 were diagnosed solely based on FCI findings in conjunction with morphologic evaluation of body fluid (1 case), fine-needle aspirate (3 cases), or excisional biopsy specimen (8 cases). The diagnoses of the remaining 7 cases were suggested strongly by FCI and confirmed by immunohistochemical analysis. FCI is useful to aid in diagnosis of ALCL, particularly along with fine-needle aspiration evaluation. ALCL with aberrant expression of myeloid antigens should not be mistaken for extramedullary myeloid tumor.