Transcriptional Analysis of Tea Plants (Camellia sinensis) in Response to Salicylic Acid Treatment
Transcriptional Analysis of Tea Plants (Camellia sinensis) in Response to Salicylic Acid Treatment
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茶树（Camellia sinensis）对水杨酸处理反应的转录分析
DOI：
10.1021/acs.jafc.2c07046
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发表时间：
2023-01-25
影响因子：
6.1
通讯作者：
Wang, Peiqiang
中科院分区：
文献类型：
--
作者：
Liu, Nana;Wang, Yueyue;Wang, Peiqiang
水杨酸(SA)是一种重要的植物激素和信号，是建立对多种病原菌和植物病害抗性所必需的。茶树中丰富的多酚类物质还可以保护植物免受生物和非生物胁迫。然而，外源SA是否会提高茶树的抗逆性，以及SA与茶多酚之间的关系，目前尚不清楚。在此，我们对茶苗进行了水杨酸处理，并进行了转录组测序。SA处理抑制了苯丙素和类黄酮类代谢途径，但促进了木质素代谢途径。SA处理后茶叶中木质素的积累增加，表明木质素可能与SA协同作用，增强和改善植物的防御和抗病能力。同时，在体外鉴定了SA诱导的黄酮类葡萄糖转移酶(CsUGT0554)，CsUGT0554特异性参与7-OH位点的糖基化。这些结果为SA对茶树幼苗的影响以及SA介导的免疫反应的分子基础提供了有价值的信息。
Salicylic acid (SA) is an important plant hormone and signal required for establishing resistance to diverse pathogens and plant diseases. The abundant polyphenols in tea plants also defend plants from biotic and abiotic stresses. However, whether exogenous SA would increase the resistance of tea plants to adversity and the relationship between SA and polyphenols are still poorly understood. Here, we carried out SA treatment on tea seedlings and performed transcriptome sequencing. SA treatment inhibited the phenylpropanoid and flavonoid metabolic pathways but promoted the lignin metabolic pathways. The increased accumulation of lignin in tea leaves after treating with SA indicated that lignin might coordinate SA, enhance, and improve plant defense and disease resistance. Simultaneously, an SA-inducible flavonoid glucosyltransferase (CsUGT0554) specifically involved in 7-OH site glycosylation was characterized in vitro. These results provided valuable information about the effects of SA on tea seedlings and the molecular basis for SA-mediated immune responses.