In situ PCR for Mycobacterium tuberculosis in endoscopic mucosal biopsy specimens of intestinal tuberculosis and Crohn disease
In situ PCR for Mycobacterium tuberculosis in endoscopic mucosal biopsy specimens of intestinal tuberculosis and Crohn disease
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DOI：
10.1309/dkkecwqwmg4j23e3
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发表时间：
2008-06-01
影响因子：
3.5
通讯作者：
Donoghue, Helen D.
中科院分区：
文献类型：
--
作者：
Pulimood, Anna B.;Peter, Shajan;Donoghue, Helen D.
结核病和克罗恩病是影响肠道的肉芽肿性疾病，具有相似的临床表现和病理特征。我们评估了使用原位聚合酶链反应（PCR），使用结核分枝杆菌复合物特异性引物IS 61 - 10，以区分这2种疾病的档案粘膜活检标本。20例结核病活检标本中6例原位PCR阳性，20例克罗恩病活检标本中1例原位PCR阳性。在3例结核病标本和克罗恩病标本中，染色局限于肉芽肿性炎症部位。在其他结核病活检标本中，阳性染色局限于炎性肉芽组织和无肉芽肿性炎症的完整粘膜病灶。克罗恩病中结核分枝杆菌DNA的存在可能是由于共存的潜伏性结核病或表明这些细菌在触发异常免疫反应中的作用。因此，如果敏感性提高，原位PCR可能有助于鉴别肠结核和克罗恩病。
Tuberculosis and Crohn disease are granulomatous disorders affecting the intestinal tract with similar clinical manifestations and pathologic features. We evaluated the use of in situ polymerase chain reaction (PCR) using Mycobacterium tuberculosis complex-specific primers for IS61 10 to differentiate these 2 disorders in archival mucosal biopsy specimens. In situ PCR was positive in 6 of 20 tuberculosis biopsy specimens and I of 20 Crohn disease biopsy specimens. Staining was localized to a site of granulomatous inflammation in 3 of the tuberculosis specimens and in the Crohn, disease specimen. In the other tuberculosis biopsy specimens, positive staining was localized to inflammatory granulation tissue and to a focus of intact mucosa without granulomatous inflammation. The presence of M tuberculosis DNA in Crohn disease could be due to coexisting latent tuberculosis or indicate a role for these bacteria in triggering an abnormal immune response. Therefore, in situ PCR is potentially useful to differentiate intestinal tuberculosis from Crohn disease, if the sensitivity is improved.