Population genetics of Ageratina adenophora using inter-simple sequence repeat (ISSR) molecular markers in China
Population genetics of Ageratina adenophora using inter-simple sequence repeat (ISSR) molecular markers in China
复制标题
DOI：
10.1080/11263500802150399
复制
发表时间：
2008-01-01
期刊：
影响因子：
2
通讯作者：
Guo, Jian-Ying
中科院分区：
文献类型：
--
作者：
Gui, Fu-Rong;Wan, Fang-Hao;Guo, Jian-Ying
本研究利用ISSR分子标记技术对紫茎泽兰（Ageratina adenophora（Spreng.）2004-2006年在中国。从100个含有二、三、四、五核苷酸重复序列的ISSR引物中筛选出20个多态性高、信息量大的引物。20条引物在64个杂草种群中扩增出479条多态性带，其中蒿属杂草具有较高的遗传多样性（H（E）=0.1541 +/- 0.0193）。沙参。根据居群间遗传距离的NJ聚类分析，将居群按地理来源分为黔西南居群、四川凉山居群、贵州西部居群、广西和重庆居群、云南南部居群、（6）四川长江流域和滇西的种群。通过Mantel检验检测到地理距离和遗传距离之间存在显著正相关（r=0.183，p=0.0012）。根据ISSR标记揭示的分化关系，推测A.紫茎泽兰在中国主要通过风和水传播。
Understanding distribution and diversity of invasive weeds is essential for the development of efficient control measures against it. In the present study, inter-simple sequence repeat (ISSR) markers were used to assess the biogeographic relationships among populations of the invasive Crofton weed (Ageratina adenophora (Spreng.)) during 2004-2006 in China. A total of 100 ISSR primers with di-, tri-, tetra- and penta-nucleotide repeats were screened, from which 20 polymorphic and informative primers were selected. Amplification of the 20 primers generated a total of 479 polymorphic bands among the 64 weed populations, and a high level of genetic diversity (H(E)=0.1541 +/- 0.0193) was detected in A. adenophora. Neighbor-joining (NJ) cluster analysis based on genetic distances among populations grouped the populations according to their geographical origin, i.e. (1) populations of southwestern Guizhou, (2) populations of Liangshan city in Sichuan, (3) populations of western Guizhou, (4) Guangxi populations plus Chongqing populations, (5) populations of southern Yunnan, and (6) populations of Yangtze River Valleys in Sichuan plus populations of western Yunnan. A significant positive correlation between geographical and genetic distance was detected by the Mantel test (r=0.183, p=0.0012). Based on the divergence relationships revealed by ISSR markers, it was assumed that A. adenophora mainly dispersed through wind and water in China.