Atomic force microscopy study of the effect of pulsed electric field on Staphylococcus epidermidis.
Atomic force microscopy study of the effect of pulsed electric field on Staphylococcus epidermidis.
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DOI：
10.1021/ac800556f
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发表时间：
2008-07
影响因子：
7.4
通讯作者：
Liu Yang;Huimin Li;Kemin Wang;W. Tan;Wenjuan Yang;Jing Zheng
中科院分区：
文献类型：
--
作者：
Liu Yang;Huimin Li;Kemin Wang;W. Tan;Wenjuan Yang;Jing Zheng
利用原子力显微镜（AFM）图像和液体中的力测量研究了脉冲电场（PEF）对表皮葡萄球菌的作用。在施加不同剂量的脉冲电场前后，原位观察细菌被膜的变化。结果表明，高压脉冲电场引起细菌肽聚糖层的变化和质膜的暴露。这一结论进一步证实了两个对照实验：溶菌酶或热对细菌包膜的影响。此外，我们的结果表明，AFM分析，包括图像和力的测量是有助于解释之间的关系的化学成分变化的细胞被膜和外部刺激。
The effect of a pulsed electric field (PEF) on Staphylococcus epidermidis was investigated by using an atomic force microscopy (AFM) image and force measurement under liquid. The changes in the bacterial envelope were probed in situ before and after applying different dosages of PEF. Our results indicated that PEF induced the changes of bacterial peptidoglycan layer and the exposure of plasma membrane. This conclusion was further confirmed by two control experiments: the effect of the lysozyme or heat on the bacterial envelope. Furthermore, our results demonstrated that AFM analysis including image and force measurement is helpful to explain the relationship between the chemical composition change of the cellular envelope and the external stimulation.