Human pituitary and placental hormones control human insulin-like growth factor II secretion in human granulosa cells.
Human pituitary and placental hormones control human insulin-like growth factor II secretion in human granulosa cells.
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DOI：
10.1073/pnas.84.9.2643
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发表时间：
1987-05
影响因子：
11.1
通讯作者：
K. Ramasharma;C. Li
中科院分区：
文献类型：
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作者：
K. Ramasharma;C. Li
用小牛血清培养的人颗粒细胞活跃地增殖18-20代，并分泌孕酮到培养基中;孕酮水平似乎随着代数的增加而下降。在无血清条件下培养的细胞分泌大量的孕酮和胰岛素样生长因子II（IGF-II）。孕酮的分泌增加了人促卵泡素，促黄体激素，绒毛膜促性腺激素，但不是由生长激素。当这些细胞受到不同浓度的人生长激素、人绒毛膜促性腺激素、人催乳素、绒毛膜促性腺激素、促卵泡素和促黄体激素的挑战时，通过特异性IGF-II RIA测量，这些细胞将IGF-II分泌到培养基中。在这些人类激素中，绒毛膜促性腺激素、促卵泡素和促黄体激素在诱导这些细胞分泌IGF-II方面最有效。当合成促黄体生成素释放激素和α-胰高血糖素-92进行测试时，只有促黄体生成素释放激素在释放IGF-II方面有效。所描述的结果表明，培养的人颗粒细胞可以增殖，并积极分泌孕酮和IGF-II到培养基中。人颗粒细胞中IGF-II的产生受到多激素复合物的影响，包括人生长激素、人绒毛膜生长催乳素和催乳素。
Human granulosa cells cultured with calf serum actively proliferated for 18-20 generation and secreted progesterone into the medium; progesterone levels appeared to decline with increase in generation number. Cells cultured under serum-free conditions secreted significant amounts of progesterone and insulin-like growth factor II (IGF-II). The progesterone secretion was enhanced by the addition of human follitropin, lutropin, and chorionic gonadotropin but not by growth hormone. These cells, when challenged to varying concentrations of human growth hormone, human chorionic somatomammotropin, human prolactin, chorionic gonadotropin, follitropin, and lutropin, secreted IGF-II into the medium as measured by specific IGF-II RIA. Among these human hormones, chorionic gonadotropin, follitropin, and lutropin were most effective in inducing IGF-II secretion from these cells. When synthetic lutropin-releasing hormone and alpha-inhibin-92 were tested, only lutropin-releasing hormone was effective in releasing IGF-II. The results described suggest that cultured human granulosa cells can proliferate and actively secrete progesterone and IGF-II into the medium. IGF-II production in human granulosa cells was influenced by a multi-hormonal complex including human growth hormone, human chorionic somatomammotropin, and prolactin.