Immunomodulatory effects of citrus fruit auraptene in vitro and in vivo
Immunomodulatory effects of citrus fruit auraptene in vitro and in vivo
复制标题
柑橘类水果 auraptene 的体外和体内免疫调节作用
DOI：
10.1016/j.jff.2012.06.005
复制
发表时间：
2012
影响因子：
5.6
通讯作者：
Sogo Nishimoto et al.
中科院分区：
文献类型：
--
作者：
Daifuku M;Nishi K;Okamoto T;Nakano H;Nishimoto S;Sugahara T;Kota Kanda et al.;Agus Budiawan Naro Putra et al.;Kosuke Nishi et al.;In-Hae Kim et al.;Sogo Nishimoto et al.
研究了auraptene在体外和体内对免疫细胞的影响。auraptene可促进人杂交瘤HB4C5细胞产生IgM。Auraptene还能刺激小鼠原代脾细胞产生IgA和IgG，以及肠系膜淋巴结淋巴细胞产生IgA和IgM。为了研究auraptene对小鼠体内免疫系统的影响，我们给小鼠口服auraptene 14d。摄取auraptene后，脾脏细胞和肠系膜淋巴结淋巴细胞的免疫球蛋白产生被显著激活。此外，在刀豆蛋白a激活的情况下，脾细胞产生IL-4、IFN-γ和TNF-α的速度加快。摄入auraptene后脾脏B细胞数量增加。
Effects of auraptene on immune cells in vitro and in vivo were examined. IgM production by human hybridoma HB4C5 cells was enhanced by auraptene. Auraptene also stimulated IgA and IgG production by mouse primary splenocytes, and IgA and IgM production by lymphocytes from mesenteric lymph nodes in vitro. To examine the effect of auraptene on immune system in vivo, auraptene was orally administered to mice for 14days. Immunoglobulin production by splenocytes and lymphocytes from mesenteric lymph nodes were significantly activated by intake of auraptene. Moreover, IL-4, IFN-γ, and TNF-α production by splenocytes was accelerated under concanavalin A-activated condition. B cell population in spleen was increased by auraptene intake.