Genome size estimates for crustaceans using Feulgen image analysis densitometry of ethanol‐preserved tissues
Genome size estimates for crustaceans using Feulgen image analysis densitometry of ethanol‐preserved tissues
复制标题
使用乙醇保存组织的富尔根图像分析密度测定法估计甲壳类动物的基因组大小
作者：
N. Jeffery;T. Gregory
甲壳类动物在系统发育和生态学上都非常多样化，但它们在现有基因组大小数据库中的代表性仍然严重不足。如果能够从乙醇保存的样本中获得基因组大小估计值，则可以促进该数据集的扩展。在这项研究中，进行了两项测试，以评估使用保存的材料生成的基因组大小数据的可靠性。首先，对基因组大小差异很大的 37 种甲壳类动物进行了基于闪冻材料和乙醇保存材料的估计结果的比较。其次，对在乙醇中储存 1-14 年的单个物种的样本进行了比较。在这两种情况下，在福尔根图像分析密度测定中使用鳃组织被证明是一种非常可行的方法。这一发现与现场收集的甲壳类动物的新研究以及基于现有收集的潜在研究直接相关。 © 2014 国际细胞计数促进会
Crustaceans are enormously diverse both phylogenetically and ecologically, but they remain substantially underrepresented in the existing genome size database. An expansion of this dataset could be facilitated if it were possible to obtain genome size estimates from ethanol‐preserved specimens. In this study, two tests were performed in order to assess the reliability of genome size data generated using preserved material. First, the results of estimates based on flash‐frozen versus ethanol‐preserved material were compared across 37 species of crustaceans that differ widely in genome size. Second, a comparison was made of specimens from a single species that had been stored in ethanol for 1–14 years. In both cases, the use of gill tissue in Feulgen image analysis densitometry proved to be a very viable approach. This finding is of direct relevance to both new studies of field‐collected crustaceans as well as potential studies based on existing collections. © 2014 International Society for Advancement of Cytometry