Collagen cross-linking in sun-exposed and unexposed sites of aged human skin.
Collagen cross-linking in sun-exposed and unexposed sites of aged human skin.
复制标题
老化人类皮肤暴露在阳光下和未暴露在阳光下的部位的胶原蛋白交联。
DOI：
10.1111/1523-1747.ep12491727
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Woodley,DT
中科院分区：
文献类型：
--
作者：
Yamauchi,M;Prisayanh,P;Haque,Z;Woodley,DT
最近描述的一种不可还原的酸热稳定化合物，组氨酸羟赖氨酸正亮氨酸（HHL），是一种从成熟皮肤组织中分离的胶原交联剂。它的丰富程度与皮肤的时间老化有关。本通讯描述了相同个体在阳光暴露（手腕）和未暴露（手腕）部位的老年人皮肤中HHL的数量。从这些部位获得了9名60岁或60岁以上的人的打孔活检。这些部位的HHL含量（摩尔/摩尔胶原蛋白）为腕关节0.13 ± 0.07，踝关节0.69 ± 0.17（平均值± SD，p < 0.001）。此外，发现用UVA（高达250 J/cm 2）和UVB（高达1 J/cm 2）急性照射交联肽对HHL结构没有影响。相同的处理显著降解了另一种不可还原的稳定胶原交联剂吡啶啉。结果表明，慢性日光暴露可能与人类真皮胶原蛋白的正常成熟障碍有关，导致HHL的数量减少。因此，真皮胶原的光老化过程不同于人类皮肤的时间老化过程。
A recently described nonreducible, acid-heat stable compound, histidinohydroxylysinonorleucine (HHL), is a collagen cross-link isolated from mature skin tissue. Its abundance is related to chronologic aging of skin. The present communication describes the quantity of HHL from aged human skin of the same individuals in sun-exposed (wrist) and unexposed (buttock) sites. Punch biopsies were obtained from these sites from nine people of age 60 or older. HHL contents (moles/ mole of collagen) at these sites were for wrist 0.13 ± 0.07 and for buttock 0.69 ± 0.17 (mean ± SD, p < 0.001). In addition, it was found that acute irradiation of the cross-linked peptides with UVA (up to 250 J/cm2) and UVB (up to 1 J/cm2) had no effect on HHL structure. The same treatment significantly degraded another nonreducible, stable collagen cross-link, pyridinoline. The results suggest that chronic sunlight exposure may be associated with an impediment to normal maturation of human dermal collagen resulting in tenuous amount of HHL. Thus, the process of photoaging in dermal collagen is different from that of chronologic aging in human skin.