Distance-independence of mitotic intrachromosomal recombination in Saccharomyces cerevisiae.
Distance-independence of mitotic intrachromosomal recombination in Saccharomyces cerevisiae.
复制标题
酿酒酵母有丝分裂染色体内重组的距离独立性。
DOI：
10.1093/genetics/124.2.263
复制
发表时间：
1990
期刊：
影响因子：
3.3
通讯作者：
Keil,RL
中科院分区：
文献类型：
--
作者：
Yuan,LW;Keil,RL
许多遗传学研究表明，同源重组的频率在很大程度上取决于重组发生的距离。我们研究了不同的重复序列长度对酿酒酵母有丝分裂染色体内重组频率的影响。我们发现，当重复序列较短时，重组频率会导致其中一个重复序列和中间序列的丢失。此外，纠正这些重复序列中包含的点突变的重组频率与重复的大小不成比例，而是显著地取决于重复序列中突变的位置。然而，有丝分裂染色体间互惠重组的频率依赖于分离标记的距离。染色体内和染色体间有丝分裂重组对同源性长度增加的反应不同，可能表明这两种情况下的重组有不同的限速步骤。这些发现对重复序列的维持和进化具有重要意义。
Many genetic studies have shown that the frequency of homologous recombination depends largely on the distance in which recombination can occur. We have studied the effect of varying the length of duplicated sequences on the frequency of mitotic intrachromosomal recombination in Saccharomyces cerevisiae. We find that the frequency of recombination resulting in the loss of one of the repeats and the intervening sequences reaches a plateau when the repeats are short. In addition, the frequency of recombination to correct a point mutation contained in one of these repeats is not proportional to the size of the duplication but rather depends dramatically on the location of the mutation within the repeated sequences. However, the frequency of mitotic interchromosomal reciprocal recombination is dependent on the distance separating the markers. The difference in the response of intrachromosomal and interchromosomal mitotic recombination to increasing lengths of homology may indicate there are different rate-limiting steps for recombination in these two cases. These findings have important implications for the maintenance and evolution of duplicated sequences.