Culture phases, cytotoxicity and protein expressions of agarose hydrogel induced Sp2/0, A549, MCF-7 cell line 3D cultures
Culture phases, cytotoxicity and protein expressions of agarose hydrogel induced Sp2/0, A549, MCF-7 cell line 3D cultures
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琼脂糖水凝胶诱导的 Sp2/0、A549、MCF-7 细胞系 3D 培养物的培养阶段、细胞毒性和蛋白质表达
DOI：
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
V. Paramesh
中科院分区：
文献类型：
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作者：
M. Ravi;S. Kaviya;V. Paramesh
细胞培养的进展正在迅速发展，一个重要的方向是在3D条件下培养细胞。我们证明了琼脂糖凝胶在获得A549、MCF-7和Sp2/0三种细胞系的三维聚集中的有效性。研究培养时相的差异、对顺铂诱导的细胞毒性的敏感性。此外，将三种细胞系的3D聚集体还原为2D培养物，并研究了2D、3D和回复培养物之间的蛋白质谱差异。使用UniProt数据库对蛋白质图谱差异的分析进一步增加了琼脂糖水凝胶在获得3D细胞培养方面的有用性。
Advancements in cell cultures are occurring at a rapid pace, an important direction is culturing cells in 3D conditions. We demonstrate the usefulness of agarose hydrogels in obtaining 3 dimensional aggregates of three cell lines, A549, MCF-7 and Sp2/0. The differences in culture phases, susceptibility to cisplatin-induced cytotoxicity are studied. Also, the 3D aggregates of the three cell lines were reverted into 2D cultures and the protein profile differences among the 2D, 3D and revert cultures were studied. The analysis of protein profile differences using UniProt data base further augment the usefulness of agarose hydrogels for obtaining 3D cell cultures.
DOI：
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发表时间：
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期刊：
The Prostate
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通讯作者：
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DOI：
10.1007/978-1-62703-125-7_13
发表时间：
2013
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
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作者：
Vidi, Pierre-Alexandre;Bissell, Mina J;Lelievre, Sophie A
通讯作者：
Lelievre, Sophie A