Polysaccharide blended whey protein isolate-(WPI) hydrogels: A physicochemical and controlled release study
Polysaccharide blended whey protein isolate-(WPI) hydrogels: A physicochemical and controlled release study
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DOI：
10.1016/j.foodhyd.2017.04.031
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发表时间：
2017-10
影响因子：
10.7
通讯作者：
Baris Ozel;S. Çıkrıkcı;O. Aydın;M. Oztop
中科院分区：
文献类型：
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作者：
Baris Ozel;S. Çıkrıkcı;O. Aydın;M. Oztop
复合乳清蛋白分离物（WPI）水凝胶的设计和表征由于其用作生物活性剂的受控递送基质而获得关注。采用常规水浴法和红外辅助微波加热法制备了黑胡萝卜提取物（BC）-黄原胶（XN）-果胶（PC）-黄蓍胶（GT）复合水凝胶。在pH7.0的磷酸盐缓冲溶液中进行释放和溶胀实验24 h。在CV XN水凝胶中观察到最高的溶胀比（SR），并且相对于不含另外的聚合物的WPI水凝胶（10.55%），仅XN水凝胶显示出SR（17.85%）的明显增加（p < 0.05）。不添加多糖的CV WPI水凝胶显示出最高的释放百分比（77.81%）。CV PC、XN和GT水凝胶的释放率分别为37.15%、32.79%和29.39%，相对于单独的WPI水凝胶能够延迟释放（p < 0.05）。MW增加了所有添加聚合物的水凝胶的释放速率。核磁共振（NMR）弛豫法用于了解样品中的聚合物-水相互作用。因此，测量每种水凝胶的横向弛豫时间（T2）和自扩散系数（SDC）。提高CV XN样品的T2和SDC值与更好的凝胶特性相关。扫描电子显微镜（SEM）图像揭示了加热和聚合物类型之间的微观结构差异。还进行了水凝胶释放行为的数学建模以估计扩散系数。此外，本研究首次介绍了微波红外加热对WPI-GT复合水凝胶的理化和缓释行为的影响。
Design and characterization of composite whey protein isolate (WPI) hydrogels are gaining interest due to their utilization as controlled delivery matrices for bioactive agents. In this study, black carrot extract (BC) loaded composite WPI hydrogels, containing xanthan (XN), pectin (PC) and gum tragacanth (GT) were prepared by conventional water bath (CV) and infrared (IR) assisted microwave heating (MW). Release and swelling experiments were conducted at pH 7.0 phosphate buffer solution for 24 h. Highest swelling ratio (SR) was observed at CV XN hydrogels and only XN hydrogels showed a distinct increase in SR (17.85%) with respect to WPI hydrogels containing no additional polymer (10.55%) (p < 0.05). CV WPI hydrogels having no added polysaccharide showed the highest release percent (77.81%). CV PC, XN and GT hydrogels with release ratios of 37.15%, 32.79% and 29.39%, respectively, were capable of retarding release with respect to sole WPI hydrogels (p < 0.05). MW increased the release rates of all polymer added hydrogels. Nuclear Magnetic Resonance (NMR) relaxometry was used to understand the polymer-water interactions in the samples. Therefore, transverse relaxation times (T2) and self-diffusion coefficients (SDC) of each hydrogel were measured. Increasing T2and SDC values of CV XN samples were associated with better gel characteristics. Scanning electron microscope (SEM) images revealed the microstructural differences between the heating and polymer types. Mathematical modelling of release behaviors of hydrogels was also conducted to estimate diffusion coefficients. Moreover, this study introduces the effects of MW-IR heating on the physiochemical and controlled release behavior of WPI-GT composite hydrogels for the first time.