Synergistic effect of anemia and red blood cells transfusion on inflammation and lung injury.
Synergistic effect of anemia and red blood cells transfusion on inflammation and lung injury.
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DOI：
10.1155/2012/924042
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发表时间：
2012
影响因子：
--
通讯作者：
Smyth SS
中科院分区：
文献类型：
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作者：
Dong A;Sunkara M;Panchatcharam M;Salous A;Selim S;Morris AJ;Smyth SS
贫血和由此产生的红细胞输注可能与不良的长期临床结局相关。为了研究相关机制，我们分析了对照组和有或无LPS诱导的全身性炎症的贫血小鼠中的炎症生物标志物和生物活性溶血磷脂介质鞘氨醇-1-磷酸（S1 P）的循环水平。急性贫血或脂多糖（LPS）的挑战单独触发的炎症标志物IL-6和角质形成细胞衍生的趋化因子（CXCL 1/KC）的循环水平的增加。此外，给贫血小鼠施用LPS降低了循环S1 P水平，增加了肺损伤和肺血管通透性。输注老化而非新鲜的红细胞（RBC）会加重肺血管渗漏。S1 P水平在小鼠RBC储存期间显著下降。在输血前用S1 P装载储存的鼠RBC部分减弱了贫血相关的急性肺血管渗漏。综上所述，我们的研究结果表明，贫血和全身炎症可以改变红细胞的S1 P缓冲能力，这表明在临床实践中减轻输血相关肺损伤的可能策略。
Anemia and resultant red blood cell transfusion may be associated with adverse long-term clinical outcomes. To investigate the mechanism(s) responsible, we profiled inflammatory biomarkers and circulating levels of the bioactive lysophospholipid mediator sphingosine-1-phosphate (S1P) in control and anemic mice with or without LPS-induced systemic inflammation. Acute anemia or lipopolysaccharide (LPS) challenge alone triggered an increase of circulating levels of the inflammatory markers IL-6 and keratinocyte-derived chemokine (CXCL1/KC). Moreover, administration of LPS to anemic mice reduced circulating S1P levels and augmented lung injury and pulmonary vascular permeability. Transfusion of aged, but not fresh, red blood cells (RBCs) worsened pulmonary vascular leak. S1P levels decline markedly during storage of mouse RBCs. Loading stored murine RBCs with S1P prior to transfusion partially attenuated anemia-associated acute pulmonary vascular leak. Taken together, our results indicate that anemia and systemic inflammation can alter the S1P buffering capacity of RBCs, suggesting possible strategies for alleviating transfusion-related lung injury in clinical practice.