Production of Laccase by Membrane-Surface Liquid Culture with Nonwoven Fabric ofCoriolus versicolor
Production of Laccase by Membrane-Surface Liquid Culture with Nonwoven Fabric ofCoriolus versicolor
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云芝无纺布膜表面液体培养生产漆酶
DOI：
10.1021/bk-2002-0830.ch009
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
M. Taniguchi
中科院分区：
文献类型：
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作者：
K. Hoshino;M. Yuzuriha;Shoichi Morohashi;S. Kagaya;M. Taniguchi
为了高效地产漆酶，并将其应用于环境污染物的生物修复，开发了非织造布膜-表面液体培养(MSLC-NF)。在以MSLC-NF为诱导剂分批生产漆酶的过程中，细胞在非织造布上生长良好，漆酶产量与普通表面培养的漆酶产量相当。在MSLC-纳滤器连续生产漆酶的过程中，以0.05~0.10h-1的稀释度提供新鲜的培养基时，可连续生产漆酶50d。将该系统应用于含0.1~1.0 mM 2，4，6-三氯苯酚废水的连续生物修复，废水中的污染物在50d内几乎完全降解。
The membrane-surface liquid culture with nonwoven fabric (MSLC-NF) was developed in order to produce effectively laccase fromCoriolus versicolorIFO4937 and to apply to the bioremediation of environmental pollutants. In the batchwise production of laccase using MSLC-NF as the naringin was added as an inducer, the cell was well growth on the nonwoven fabric and the amount production of laccase was as the same as that by the normal surface culture. In the continuous production of laccase using MSLC-NF by supplying the fresh medium at a dilution rate of 0.05-0.10 h-1, laccase could be produced successively for 50 days. Further, when this system was applied to the continuous bioremediation of the wastewater contained 0.1-1.0 mM of 2,4,6-trichlorophenol, the pollutant in the wastewater was almost completely degraded for 50 days.