Dysregulated miR-645 affects the proliferation and invasion of head and neck cancer cell.
Dysregulated miR-645 affects the proliferation and invasion of head and neck cancer cell.
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miR-645失调影响头颈癌细胞的增殖和侵袭
DOI：
10.1186/s12935-015-0238-5
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发表时间：
2015
影响因子：
5.8
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Sun Q;Chen S;Zhao X;Yan M;Fang Z;Wang H;Zhao J;Sun M;Han X;Chen W;Li X
背景与目的：miRNAs表达异常在多种恶性肿瘤中起重要作用。然而，阐明miRNA在癌症生物学中的作用，特别是在上皮癌中，仍然是一个正在进行的过程。本研究旨在寻找在头颈部鳞状细胞癌（HNSCC）发生发展过程中差异表达的miR-645，并探讨其生物学功能。方法：采用Kaplan-Meier生存分析法分析候选miRNA表达水平与临床病理参数之间的关系。结果：34例有淋巴结转移的HNSCC组织中，miR-645的表达水平为0.54 ± 0.12，而28例无淋巴结转移的HNSCC组织中，miR-645的表达水平为0.22 ± 0.05（p = 0.017）。在HNSCC患者中，较高水平的miR-645表达与较差的总生存期显著相关（p = 0.04）。结论：miR-645在HNSCC的侵袭和转移中起重要作用，其表达与患者的总体生存率相关。
Background and purpose:Dysregulated miRNAs play an important role in many malignant tumors. However, elucidating the roles of miRNAs in cancer biology, especially in epithelial cancers, remains an ongoing process. In this study, we identified the differentially expressed miR-645 in the progressing of head and neck squamous cell carcinoma (HNSCC) and investigated its biological function.Methods:The association between clinicopathological parameters and the expression levels of the candidated miRNAs were analyzed by using the Kaplan-Meier survival analysis. The cell growth, invasion and migration potential, and clone formation were observed to detect the functions of the miRNAs in HNSCC cells.Results:In the 34 HNSCC tissues with lymph node metastasis, the expression level of miR-645 was 0.54 ± 0.12, and the expression level was 0.22 ± 0.05 in the 28 tissues with non lymph node metastasis (p = 0.017). In patients with HNSCC, higher level of miR-645 expression significantly correlates with worse overall survival (p = 0.04). Ectopic expression of miR-645 promoted cell invasion and migration.Conclusions:miR-645 play a key role in cell invasion and metastasis and their expression correlates with overall survival in the patients with HNSCC.