A new inhibitor of metalloproteinases from chicken: ChIMP-3. A third member of the TIMP family.
A new inhibitor of metalloproteinases from chicken: ChIMP-3. A third member of the TIMP family.
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DOI：
10.1016/s0021-9258(18)41928-x
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发表时间：
1992-08
期刊：
影响因子：
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通讯作者：
N. Pavloff;P. Staskus;N. Kishnani;Susan P. HawkesQ
中科院分区：
文献类型：
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作者：
N. Pavloff;P. Staskus;N. Kishnani;Susan P. HawkesQ
我们报道了一种新的由鸡胚胎成纤维细胞产生的金属蛋白酶抑制因子(CHAMP-3)的基因克隆和一级结构。黑猩猩-3，以前被称为21-kDa蛋白，是五只黑猩猩(鸡肉金属蛋白酶抑制因子)之一。在本文中，我们报道了在最丰富的三种黑猩猩中，黑猩猩-3和黑猩猩-a是细胞外基质成分，而黑猩猩-2存在于细胞条件培养液中。用N-糖苷酶-F处理黑猩猩-3和黑猩猩-a，表明黑猩猩-a是N-糖基化的，而黑猩猩-3不是。黑猩猩-3推导的氨基酸序列预测了一种蛋白质，其性质与实验测量一致。对来自不同物种的金属蛋白酶组织抑制因子(TIMP)家族的两个成员TIMP-1和TIMP-2的序列比对分析表明，CHAMP-3是一个相关但不同的蛋白质。缺乏与抗TIMP-1和抗TIMP-2抗体的显著结合支持这一结论。基于这些数据，其特殊的溶解性，以及它在基质中的独特位置，我们认为CHAMP-3是金属蛋白酶抑制剂家族中的一个新成员，即TIMP-3。
We report cDNA cloning and primary structure of a new metalloproteinase inhibitor (ChIMP-3) produced by chicken embryo fibroblasts. ChIMP-3, formerly called the 21-kDa protein, is one of five ChIMPs (Chicken Inhibitor of MetalloProteinases). In this paper, we report that of the three most abundant ChIMPs, ChIMP-3 and ChIMP-a are extracellular matrix components, whereas ChIMP-2 is found in the media conditioned by the cells. Treatment of ChIMP-3 and ChIMP-a with N-glycosidase-F indicates that ChIMP-a is N-glycosylated whereas ChIMP-3 is not. The deduced amino acid sequence of ChIMP-3 predicts a protein whose properties are consistent with experimental measurements. Analysis of sequence alignments with the two previously described members of the TIMP (tissue inhibitor of metalloproteinases) family, TIMP-1 and TIMP-2, from various species indicates that ChIMP-3 is a related but distinct protein. This conclusion is supported by lack of significant binding with anti-TIMP-1 and anti-TIMP-2 antibodies. Based on these data, its unusual solubility properties, and its exclusive location in the matrix, we propose that ChIMP-3 is a new member of this family of metalloproteinase inhibitors, a TIMP-3.