Metabolic engineering of the mevalonate and non-mevalonate isopentenyl diphosphate-forming pathways for the production of health-promoting isoprenoids in tomato
Metabolic engineering of the mevalonate and non-mevalonate isopentenyl diphosphate-forming pathways for the production of health-promoting isoprenoids in tomato
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DOI：
10.1111/j.1467-7652.2004.00091.x
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发表时间：
2005-01-01
影响因子：
13.8
通讯作者：
Bramley, PM
中科院分区：
文献类型：
--
作者：
Enfissi, EMA;Fraser, PD;Bramley, PM
利用3-羟甲基戊二酰辅酶A(HMGR-1)和1-脱氧-D-木糖-5-磷酸合成酶(DXS)基因分别对番茄甲戊酸(MVA)和甲基赤霉醇-4-磷酸(MEP)途径进行了遗传操作，形成了异戊烯基二磷酸(IPP)。在花椰菜花叶病毒(CaMV)35S构成启动子的控制下，含有来自拟南芥的HMGR-1的转基因植株含有高达2.4倍的植物甾醇，但IPP衍生的异戊二烯类化合物在质体中没有改变。含有以番茄DXS转移序列为靶点的细菌DXS的转基因株系，类胡萝卜素含量增加(1.6倍)，并在下一代遗传。植酸烯和β-胡萝卜素的增幅最大(分别是2.4倍和2.2倍)。超塑性异戊二烯类化合物在这些品系中没有受到影响。这些数据涉及异戊二烯生物合成途径的调节、划分和操纵，以及它们与植物生物技术的相关性。
The genetic manipulation of both the mevalonic acid (MVA) and methylerythritol-4-phosphate (MEP) pathways, leading to the formation of isopentenyl diphosphate (IPP), has been achieved in tomato using 3-hydroxymethylglutaryl CoA (hmgr-1) and 1-deoxy-D-xylulose-5-phosphate synthase (dxs) genes, respectively. Transgenic plants containing an additional hmgr-1 from Arabidopsis thaliana, under the control of the cauliflower mosaic virus (CaMV) 35S constitutive promoter, contained elevated phytosterols (up to 2.4-fold), but IPP-derived isoprenoids in the plastid were unaltered. Transgenic lines containing a bacterial dxs targeted to the plastid with the tomato dxs transit sequence resulted in an increased carotenoid content (1.6-fold), which was inherited in the next generation. Phytoene and beta-carotene exhibited the greatest increases (2.4- and 2.2-fold, respectively). Extra-plastidic isoprenoids were unaffected in these lines. These data are discussed with respect to the regulation, compartmentalization and manipulation of isoprenoid biosynthetic pathways and their relevance to plant biotechnology.