Hepatoprotective effect of blocking N-methyl-D-aspartate receptors in male albino rats exposed to acute and repeated restraint stress
Hepatoprotective effect of blocking N-methyl-D-aspartate receptors in male albino rats exposed to acute and repeated restraint stress
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DOI：
10.1139/cjpp-2016-0556
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发表时间：
2017-06-01
影响因子：
2.1
通讯作者：
Hassan, Sherif Sabry
中科院分区：
文献类型：
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作者：
Amin, Shaimaa Nasr;El-Aidi, Ahmed Amro;Hassan, Sherif Sabry
除大脑外，压力还会影响许多器官，包括肝脏。我们评估了在急性和重复的束缚应激中用美金刚阻断 N-甲基-D-天冬氨酸 (NMDA) 谷氨酸受体对肝脏的影响。 42只雄性白化大鼠分为7组；对照组、急性束缚应激（ARS）、ARS + 美金刚、重复束缚应激、重复束缚 + 美金刚和阳性对照组。我们测量了血清铁、锌、丙氨酸转移酶和阿斯巴甜转移酶、肝丙二醛、肿瘤坏死因子-α (TNF-α)、谷胱甘肽过氧化物酶、超氧化物歧化酶、金属硫蛋白含量、锌转运蛋白 ZRT/IRT 样蛋白 14 mRNA 表达和铁调素表达。我们通过组织学染色和免疫染色对胶质纤维酸性蛋白和突触素表达进行了组织病理学评估，这两者都是肝星状细胞（HSC）激活的标志物。 ARS 和反复应激都会增加肝细胞损伤、氧化应激和 HSC 激活的标志物。用美金刚阻断 NMDA 可在急性和重复的束缚应激中发挥保肝作用，并减少肝细胞损伤、氧化应激和 HSC 活化。
Stress affects many organs in addition to the brain, including the liver. We assessed the effects on the liver of blocking N-methyl-D-aspartate (NMDA) glutamate receptors with memantine in acute and repeated restraint stress. Forty-two male albino rats were divided into 7 groups; control, acute restraint stress (ARS), ARS + memantine, repeated restraint stress, repeated restraint + memantine, and positive control groups. We measured serum iron, zinc, alanine transferase and aspartame transferase, hepatic malondialdehyde, tumor necrosis factor-alpha (TNF-alpha), glutathione peroxidase, superoxide dismutase, metallothionein content, zinc transporter ZRT/IRT-like protein 14 mRNA expression, and hepcidin expression. We conducted a histopathological evaluation via histological staining and immunostaining for glial fibrillary acidic protein and synaptophysin expression, both of which are markers of hepatic stellate cell (HSC) activation. Both ARS and repeated stress increased markers of hepatic cell injury, oxidative stress, and HSC activation. Blocking NMDA with memantine provided a hepatoprotective effect in acute and repeated restraint stress and decreased hepatic cell injury, oxidative stress, and HSC activation.