Imaging of astrocytic activity in living rodents.
Imaging of astrocytic activity in living rodents.
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活体啮齿动物星形胶质细胞活动的成像。
DOI：
10.1007/978-1-62703-785-3_12
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
*Hirase H
中科院分区：
文献类型：
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作者：
Takata N;Shinohara Y;Ohkura M;Mishima T;Nakai J;*Hirase H
越来越多的体外实验证据支持星形胶质细胞和神经元之间的双向相互作用，表明神经胶质参与大脑中神经元的信息处理。胞质钙离子（Ca2+）浓度的升高被认为是胶质传递的重要因素；然而，体内大脑皮层星形胶质细胞Ca2+动力学的研究直到最近才成为可能。在这里，我们描述了一套程序，以监测Ca2+浓度波动的星形胶质细胞群体或在星形细胞过程中使用体内双光子显微镜。通过电极同时记录神经元活动和成像同时记录星形胶质细胞活动是揭示活体啮齿动物大脑中神经元-神经胶质相互作用本质的一种很有前途的方法。
Mounting evidence from in vitro experiments supports bidirectional interactions between astrocytes and neurons, suggesting glial involvement in neuronal information processing in the brain. Elevation of the cytosolic calcium ion (Ca2+) concentration has been suggested to be important for gliotransmission; however, the study of Ca2+dynamics in cerebral cortical astrocytes in vivo became possible only recently. Here, we describe a set of procedures to monitor Ca2+concentration fluctuations in a population of astrocytes or in astrocytic processes using in vivo two-photon microscopy. Simultaneous recording of neuronal activity by electrodes and astrocytic activity by imaging is a promising way to reveal the nature of neuron–glia interactions in the brain of living rodents.