Division of an Iliac Crest Bone Biopsy Specimen to Allow Histomorphometry, Immunohistochemical, Molecular Analysis, and Tissue Banking: Technical Aspect and Applications.
Division of an Iliac Crest Bone Biopsy Specimen to Allow Histomorphometry, Immunohistochemical, Molecular Analysis, and Tissue Banking: Technical Aspect and Applications.
复制标题
Iliac Crest骨活检标本的划分，以允许组织形态计量学，免疫组织化学，分子分析和组织库：技术方面和应用。
作者：
Picard S;Mayemba CN;Ung RV;Martel S;Mac-Way F
评估慢性肾脏病(CKD)的骨并发症通常需要进行骨活检，对其进行分析可以完善骨缺损的诊断。骨组织形态计量学仍然是定量评估慢性肾脏病患者骨健康的参考方法，而免疫组织化学和其他分子生物学分析是研究信号通路中断的不可或缺的工具。传统上，整个髂骨活检包括在甲基丙烯酸甲酯(MMA)中，仅用于骨组织形态计量学，以描述静态、动态和结构参数。因此，对骨组织进行进一步的分子分析或组织库的需要将需要进行第二次活组织检查，因为MMA中的包裹物阻止了高质量核酸的提取。在这项工作中，我们描述了一种简单的方法，将单个髂骨骨活检分成多个部分。这使得可以同时评估组织学、免疫组织化学、生物分子分析和组织库，同时为组织形态计量学保留相同的骨表面积。《美国骨与矿物研究学会》作者。JBMR Plus由威利期刊有限责任公司代表美国骨骼和矿物研究学会出版。
The evaluation of bone complications in chronic kidney disease (CKD) often requires a bone biopsy, the analysis of which can refine the diagnosis of bone defects. Bone histomorphometry performed on sections of the iliac crest biopsy remains the reference procedure for the quantitative assessment of bone health in CKD patients, whereas immunohistochemistry and other molecular biology analyses are indispensable tools for studying the disrupted signaling pathways. Traditionally, the whole iliac crest biopsy was included in methyl‐methacrylate (MMA) and was exclusively used for bone histomorphometry to describe static, dynamic, and structural parameters. Therefore, further molecular analysis of the bone tissue or the need for tissue banking would require a second biopsy to be made, because inclusion in MMA prevents the extraction of good‐quality nucleic acids. In this work, we describe a simple approach to divide a single iliac crest bone biopsy into multiple parts. This allows for simultaneous assessments of histology, immunohistochemistry, biomolecular analysis, and tissue banking while preserving the same bone surface area for histomorphometry. © 2020 American Society for Bone and Mineral Research © 2020 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.